植物单细胞转录组测序研究进展

Research Progress in Single-Cell RNA-Seq of Plant

单细胞转录组测序是一种在单细胞水平上研究基因表达的技术。多孔板法和液滴法是目前应用于植物研究的两类主要的单细胞转录组技术。首先概述了植物单细胞转录组测序的技术原理和数据分析流程,然后介绍了植物单细胞转录组的研究进展,重点阐述了单细胞转录组测序技术在鉴定植物细胞类型、揭示细胞演化轨迹和构建细胞间调控网络中的应用。单细胞转录组测序技术可为植物领域的研究提供新视角,有助于识别和理解复杂组织中关键的细胞生物学过程。

Single-cell RNA-Seq(scRNA-seq)is a technology to analyze gene expression at single cell level.Currently PCR plate-based and droplet-based protocols are 2 major popular ones used in the studies of plant.Here,we firstly outline the technical principles and data analysis procedure of plant scRNA-seq,and then introduce its research status,focusing on how scRNA-seq can be used to detect distinct plant cell types,to reveal cell evolution trajectories and to construct inter-cell regulatory networks.ScRNA-seq may provide a new perspective on plant research,and help to understand and identify critical cellular biological processes in complex tissues.