单细胞转录组测序技术在心脏发育、疾病以及医学中的应用

Applications of Single-cell RNA Sequencing in Heart Development,Disease and Medicine

单细胞转录组测序(Single-cell RNA sequencing,scRNA-seq)可以在单细胞水平描绘出每个细胞同一基因的表达量在不同细胞间的表达水平差异,使得在单细胞水平重新认识各种组织器官成为可能。目前对心脏的测序研究正从传统的普通转录组水平过渡到单细胞水平,对小鼠和人的心脏的测序陆续地发表出来。概述了scRNA-seq在心脏发育、疾病以及医学中的应用,讨论了scRNA-seq技术在胚胎心脏发育、心脏细胞的异质性以及在心脏血管方面、多能干细胞分化心血管细胞模型和先天性心脏畸形的进展和存在问题,对scRNA-seq技术对心脏发育、心脏再生、心脏病和单细胞个性化医疗等方面作出展望。

Single-cell RNA sequencing(scRNA-seq)can depict the difference in the expression level of the same gene between different cells at the single-cell level,enabling a new understanding of various tissues and organs at the single-cell level.At present,the sequencing research of the heart is transitioning from the bulk RNA-seq to the scRNA-seq,and research articles on the sequencing of mice’s and human’s heart have been published one after another.This article reviews the application of scRNA-seq in heart development,disease and medicine,and discusses scRNA-seq in embryonic heart development,cell heterogeneity of heart,pluripotent stem cells derived cardiovascular cell models,and congenital heart malformation.Finally,we conclude with prospects of scRNA-seq technology for heart development,heart regeneration,heart disease and single-cell precision medicine.