摘要
RNA干扰(RNAi)是生物体内源基因发生转录后特异性降解的一种生理现象,广泛存在于生物体内。RNAi主要由小干扰RNA诱发阻碍目的基因的翻译或转录,造成目标信使RNA沉默。RNAi具有高效、特异性强等优点,被广泛应用于昆虫基因功能研究,并显示出了开发新型病虫害管理策略的巨大潜力。主要阐述了RNAi的沉默机制,双链RNA转入昆虫体内的几种方式,以及RNAi技术在不同目昆虫中研究的最新进展。最后,对RNAi技术存在的不足之处进行了简单总结,还对RNAi技术在害虫防治中的应用进行了展望,以期为该技术广泛应用于农业害虫防治提供理论支持。
RNA interference(RNAi)is a physiological phenomenon of post-transcriptional degradation of endogenous genes in organism,and it is widely present in organisms.RNAi is mainly induced by short/small RNA to hinder the translation or transcription of the target gene,and which cause the target messenger RNA to be silence.RNAi has the advantages of high efficiency and specificity,thus it is widely used in insect gene function research and has shown great potential for development of novel pest management strategies.This review mainly expounds the silencing mechanism of RNAi,several ways of transferring double-stranded RNA into insects,and the latest research progress of RNAi technology in insects of different orders.Finally,the paper briefly summarizes the shortcomings of RNAi technology,and prospects the application of RNAi technology in pest control,with a view to providing theoretical support for the widespread application of this technology in agricultural pest control.
作者
徐雪亮
王奋山
刘子荣
范琳娟
季香云
蒋杰贤
姚英娟
XU Xue-liang;WANG Fen-shan;LIU Zi-rong;FAN Lin-juan;JI Xiang-yun;JIANG Jie-xian;YAO Ying-juan(Applied Agricultural Micro-organism Research,Jiangxi Academy of Agricultural Sciences,Nanchang 330200;Eco-environment Protection Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403)
出处
《生物技术通报》
CAS
CSCD
北大核心
2021年第1期255-261,共7页
Biotechnology Bulletin
基金
国家自然科学基金项目(地区基金)(31660514)
江西省农业科学院创新基金(青年基金)(2016CQN005)。
关键词
RNA干扰
昆虫
转运机制
害虫防治
RNA interference
insect
transfer mechanism
pest control
