白花蛇舌草多糖对Lewis肺癌小鼠肿瘤抑制作用及机制研究

Anti-tumor Effect and Mechanism of Hedyotis Diffusa Polysaccharide on Lewis Lung Cancer Mice

目的:白花蛇舌草多糖对Lewis肺癌小鼠肿瘤抑制作用及机制研究。方法:选取C57BL/6J小鼠,建立Lewis肺癌模型,随机分为空白组、模型组、顺铂组(6 mg·kg^-1)、白花蛇舌草多糖高、中、低剂量(200 mg·kg^-1、100 mg·kg^-1、50 mg·kg^-1)组,各组小鼠给予相应药物灌胃,连续给药21 d。取肿瘤组织称重,测定肿瘤抑制率;腹主动脉取血检测血清中白细胞介素-2(interleukin-2,IL-2)、白细胞介素-10(interleukin-10,IL-10)、肿瘤坏死因子(tumor necrosis factor alpha,TNF-α)、干扰素-γ(interferon gamma,TFN-γ)、血管内皮生长因子(vascular endothelial growth factor,VEGF)、缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)、基质金属蛋白酶-2(matrix metalloproteinase 2,MMP-2)含量;RT-PCR法检测肿瘤组织中VEGF、HIF-1α、Bcl-2和Bax的基因表达量。结果:白花蛇舌草多糖各剂量组均可明显升高模型小鼠血清中IL-2、TNF-α、TFN-γ水平(P<0.05),降低IL-10、VEGF、HIF-1α、MMP-2水平(P<0.05),同时可明显降低模型小鼠肿瘤组织中VEGF mRNA、HIF-1α、Bcl-2 mRNA表达(P<0.05),升高Bax mRNA表达(P<0.05),从而抑制肿瘤瘤体的增长。结论:白花蛇舌草多糖对小鼠Lewis肺癌组织具有明显的抑制作用。

Objective:To observe the anti-tumor effect and mechanism of Hedyotis diffusa polysaccharide on Lewis lung cancer mice.Methods:C57 BL/6 J mice were selected to establish Lewis lung cancer models.The animals were randomly divided into blank group,model group,cisplatin group(6 mg·kg^-1),and Hedyotis diffusa polysaccharide(200 mg·kg^-1,100 mg·kg^-1,50 mg·kg^-1)groups.Animals in each group were given corresponding drugs by gavage for 21 days.Tumor tissues from each group of animals were weighed to determine the tumor inhibition rate;blood was collected from the abdominal aorta to detect serum interleukin-2(IL-2)and interleukin-10(IL-10),tumor necrosis factor alpha(TNF-α),interferon-γ(TFN-γ),vascular endothelial growth factor(VEGF),hypoxia inducible factor-1α(HIF-1α),matrix metalloproteinase 2(MMP-2)content.RT-PCR method to detect the expression of VEGF,HIF-1α,Bcl-2 and Bax mRNA in tumor tissues.Results:Each dose group of Hedyotis diffusa polysaccharide can significantly increase the serum levels of IL-2,TNF-α,TFN-γ,and decrease the levels of IL-10,VEGF,HIF-1α,and MMP-2 in model animals(P<0.05).At the same time,it can reduce the expression of VEGF mRNA,HIF-1αmRNA and Bcl-2 mRNA in tumor tissues of model animals,increase the expression of Bax mRNA(P<0.05),and inhibit the growth of tumor tumors.Conclusion:Hedyotis diffusa polysaccharide has obvious inhibitory effect on Lewis lung cancer tissue in mice.