急性饮酒对青年人记忆功能相关脑区CBF及OEF影响的多模态磁共振研究

A multimodal magnetic resonance study of the effects of acute alcohol on CBF and OEF in memory-related brain regions in young people

目的利用三维伪连续式动脉自旋标记(three-dimensional pseudo-continuous arterial spin labeling,3D pCASL)和非对称基于自旋回波的回波平面成像(asymmetric spine echo-based echo plane imaging,ASE EPI)研究急性饮酒对健康青年人记忆功能相关脑区脑血流灌注(cerebral blood flow,CBF)、氧摄取分数(oxygen extraction fraction,OEF)的影响,分析不同剂量急性饮酒后健康志愿者记忆功能相关脑区CBF与OEF的关系。材料与方法29名健康志愿者分别摄入不同剂量的白酒,饮酒后,抽血检测血液酒精浓度(blood alcohol concentration,BAC),按照BAC≥0.04%、0.06%、0.08%分为低、中、高浓度组。所有受试者分别于饮酒前及饮酒后30 min进行记忆功能评价并行MRI扫描,饮酒前数据作为对照组。对饮酒前、后左侧额中回、顶叶、枕叶皮层、双侧扣带回、海马、杏仁核及小脑半球等记忆功能相关脑区的CBF和OEF进行比较分析。结果(1)饮酒后30 min与饮酒前相比,左侧额中回、顶叶、枕叶皮层、双侧扣带回、海马、杏仁核CBF增加(P<0.05),双侧小脑半球CBF减低(P<0.05)。饮酒后30 min,不同剂量组间左侧额中回、顶叶、枕叶皮层、双侧扣带回、海马、杏仁核的平均CBF差异有统计学意义(P<0.05)。(2)饮酒后30 min与饮酒前相比,左侧额中回、顶叶、枕叶皮层、双侧扣带回、海马OEF减低(P<0.05),双侧小脑半球OEF增加(P<0.05)。饮酒后30 min,不同剂量组间左侧额中回、顶叶、枕叶皮层、双侧扣带回、海马、小脑半球的平均OEF差异有统计学意义(P<0.05)。结论饮酒后记忆功能相关脑区的CBF、OEF呈负相关关系,脑组织氧供给与氧利用处于动态平衡。饮酒后记忆功能相关脑区脑血流和脑代谢信息的改变,在揭示酒精对记忆功能相关脑区的作用机制有一定的意义。

Objective:Three-dimensional pseudo-continuous arterial spin labeling(3D pCASL)and asymmetric spine echo-based echo plane imaging(ASE EPI)were used to study the effects of different doses of acute alcohol on cerebral blood flow(CBF)and oxygen extraction fraction(OEF)in the memory-related brain regions of healthy young people,and to analyze the relationship between CBF and OEF in memory-related brain regions after different doses of acute alcohol consumption.Materials and Methods:Twenty-nine young volunteers were fed with different doses of Chinese alcohol.After drinking,blood alcohol concentration(BAC)was measured.BAC≥0.04%was the low concentration group,BAC≥0.06%was the medium concentration group,and BAC≥0.08%was the high concentration group,respectively.All subjects were examined by the memory function test and brain MRI before drinking and 30 minutes after drinking.The data before drinking was as the control group.The values of CBF and OEF in left middle frontal gyrus,parietal lobe,occipital cortex,bilateral cingulate gyrus,hippocampus,amygdala and bilateral cerebellar hemisphere were compared before and after drinking respectively.Results:(1)Compared with pre-drinking,30 minutes after drinking,the CBF of left middle frontal gyrus,parietal lobe,occipital cortex,bilateral cingulate gyrus,hippocampus,amygdala increased(P<0.05),and the CBF of bilateral cerebellar hemisphere decreased(P<0.05).The difference of CBF within mild,moderate and severe dose groups was statistically significant(P<0.05)by multiple comparison of LSD-t test.(2)Compared with pre-drinking,30 minutes after drinking,the OEF of left middle frontal gyrus,parietal lobe,occipital cortex,bilateral cingulate gyrus,hippocampus,amygdala decreased(P<0.05),and the OEF of bilateral cerebellar hemisphere increased(P<0.05).The difference of OEF within mild,moderate and severe dose groups was statistically significant(P<0.05)by multiple comparison of LSD-t test.Conclusions:After acute alcohol,the CBF and OEF of the memory-related brain regions showed a negative correlation,and the brain oxygen supply and utilization were in dynamic balance.The changes of cerebral blood flow and cerebral metabolism information of memory-related brain regions after acute alcohol have certain significance in revealing the mechanism of alcohol on the memory-related brain regions.