大枣多糖对小鼠淋巴细胞免疫调节作用的研究

Research on the Immunoregulatory Effect of Jujube Polysaccharide on Lymphocyte in Mice

研究大枣多糖对小鼠淋巴细胞的免疫调节作用。将小鼠淋巴细胞无菌分离,制备细胞悬液,设计空白组、阳性对照组(左旋咪唑)以及不同质量浓度的大枣多糖组(终浓度为20、40、80、160和320μg/mL)。按照MTT法观察大枣多糖对小鼠淋巴细胞增殖的影响情况;采用ELISA法观察大枣多糖对小鼠淋巴细胞上清液中IL-2、IL-6、IL-10、IL-12水平的影响;QRT-PCR检测大枣多糖对小鼠淋巴细胞IL-2、IL-6、IL-10、IL-12信使核糖核酸(mRNA)表达的影响。在20~320μg/mL浓度范围内,大枣多糖能促进小鼠淋巴细胞增殖,同时提高细胞因子的分泌量,进而促进细胞因子IL-2、IL-6、IL-10、IL-12 mRNA表达。在20~320μg/mL浓度范围内,大枣多糖通过诱导淋巴细胞增殖、淋巴细胞因子IL-2、IL-6、IL-10、IL-12分泌以及mRNA表达,从而提高机体免疫功能。

To study the immunomodulatory effect of jujube polysaccharide on mouse lymphocytes.Mouse lymphocytes were sterile separated,cell suspension was prepared,and the blank group,the positive group(levamisole)and the jujube polysaccharide group with different mass concentrations(final concentrations of 20,40,80,160 and 320 livingμg/ml)were designed.The effect of jujube polysaccharide on the proliferation of mouse lymphocytes was observed by MTT method.The effects of jujube polysaccharide on the levels of interleukin-2(IL-2),interleukin-6(IL-6),interleukin-10(IL-10)and interleukin-12(IL-12)in the supernatant of mouse lymphocytes were observed by ELISA.The effect of jujube polysaccharide on the expression of IL-2,IL-6,IL-10 and IL-12 mRNA in mouse lymphocytes was detected by QRT-PCR.Jujube polysaccharide could promote the proliferation of mouse lymphocytes and the secretion of cytokines in the range of 20-320μg/ml,which further promoted the expression of cytokines IL-2 and IL-6,as well as IL-10 and IL-12 mRNA.Conclusion:jujube polysaccharide enhances immune function by inducing lymphocyte proliferation and the secretion and mRNA expression of lymphocyte cytokines IL-2 and IL-6,as well as IL-10 and IL-12.