三棱抗胃癌的潜在药物靶点及作用机制研究

Study on potential drug targets and mechanism of sparganium in treating gastric cancer

目的基于网络药理学,寻找三棱可能抑制胃癌的有效活性成分,分析其抗胃癌的分子机制。方法收集三棱药物有效活性成分、胃癌治疗靶点,构建“中药三棱-化合物-靶点-胃癌”网络及PPI网络,进行GO富集和KEGG通路分析,预测三棱抗胃癌调控的信号通路及生物学过程。结果筛选出三棱的5个主要有效活性成分(反式-11-二十碳烯酸、常春藤皂苷元、β-谷甾醇、芒柄花素、豆甾醇),28个与胃癌共同作用的蛋白靶点,2条三棱参与抗胃癌作用的主要信号通路,包括凋亡通路、p53通路。结论三棱抗胃癌潜在有效活性成分有5个,抗胃癌机制可能与p53通路及凋亡通路的调控有关。

Objective To search for the potential efficient active components of sparganium which could inhibit gastric cancer and study the molecular mechanism of anti-gastric cancer based on network pharmacology.Methods Sparganium drug effective active ingredient and therapeutic targets for gastric cancer were collected.Sparganium-Compound-Target-Gastric cancer network and protein-protein interaction(PPI)network were developed.Gene ontology(GO)enrichment analysis and Kyoto encyclopedia of genes and genomes(KEGG)pathway analysis were performed to predict the regulatory signaling pathways and biological processes of sparganium in the treatment of gastric cancer.Results Five main active components of sparganium(trans-11-eicosenoic acid,ivy saponin,β-sitosterol,formononetin and stigmasterol),28 protein targets co-acting with gastric cancer were screen out.And the two main signaling pathways were involved in anti-gastric cancer,including apoptosis pathway and p53 pathway.Conclusion There are 5 potential effective active components of sparganium against gastric cancer,and the mechanism of anti-gastric cancer may be related to the regulation of p53 pathway and apoptotic pathway.