幼年大鼠和老年大鼠肠道菌群结构及多样性分析

Structure and diversity of intestinal flora in infant and aged rats

为研究幼年大鼠和老年大鼠肠道菌群的结构及多样性,对7只幼年大鼠(1.5月龄)和5只老年大鼠(24月龄)肠道菌群的构成进行了比较,收集相同环境下饲养的幼年组和老年组大鼠的粪便,对其进行16S核糖体RNA基因V3+V4区的测序以分析肠道菌群的构成。结果表明:幼年组大鼠个体间肠道菌群结构差异较大,而老年组大鼠相对较集中,两组大鼠肠道菌群在门级水平上相对丰度相似;幼年组大鼠乳杆菌科下的微生物所占丰度高于老年组大鼠(p<0.05),而梭菌目瘤胃菌科下微生物丰度低于老年组大鼠(p<0.05);老年组大鼠的肠道生物多样性高于幼年组大鼠,老年组大鼠的肠道菌群多样性较高,菌群种类丰富,但其中有害菌比例显著升高。该结果填补了不同年龄大鼠肠道菌群结构研究的空白,为改善实验用大鼠的健康状况以延长其寿命提供一定的理论基础。

In order to study the structure and diversity of intestinal flora between infant and aged rats,the composition of intestinal flora in 7 infant rats(1.5 months old)and 5 aged rats(24 months old)was compared.Feces of infant and aged rats fed under the same environment were collected.The V3+V4 region of 16 S ribosomal RNA gene was sequenced to analyze the composition of intestinal flora.The results showed that the intestinal flora structure of the infant group was significantly different,while that of the aged group was relatively concentrated.The relative abundance of intestinal flora in the two groups was similar at the phylum level.The abundance of microorganism under lactobacillus in infant rats was higher than that in aged rats(p<0.05),while that of microorganism under clostridium rumen bacteria was lower than that in aged rats(p<0.05).The intestinal biodiversity of the aged rat group was higher than that of the infant rat group.The intestinal flora diversity of the aged rat group was higher,and the species of intestinal flora were rich,but the proportion of harmful bacteria significantly increased.It makes up for the blank of the research on intestinal flora structure of rats at different age,and provides certain reference for improving the health conditions of experimental rats to extend their lives.