肝细胞肝癌中Piwil2表达与临床病理特征及预后的关系

Expression of Piwil2 in hepatocellular carcinoma and relationship with clinicopathologic characters and prognosis

目的探讨肝细胞肝癌(hepatocellular carcinoma,HCC)中Piwil2的表达及其与HCC的发生与演进、临床病理特征、预后的关系。方法利用组织芯片技术和免疫组化PV 6000法检测163例HCC组织及对应癌旁非瘤组织中Piwil2的表达,并进行统计学分析。结果HCC组织中Piwil2的高表达率为47.85%(78/163),而癌旁非瘤组织中未发现高表达病例,两者差异有统计学意义(χ^2=102.532,P<0.001)。Piwil2表达与HCC病理分级、TNM分期、包膜侵犯、脉管侵犯均有关(P均<0.05),病理分级Ⅲ+Ⅳ级、TNM分期Ⅲ+Ⅳ期的患者高表达率高于Ⅰ+Ⅱ级、Ⅰ+Ⅱ期的患者(73.33%vs 26.14%,P<0.001;68%vs 44.2%,P=0.028)。Kaplan-Meier预后生存分析结果显示,Piwil2高表达组患者的总生存期和无瘤生存期较低表达组短(P均<0.05);有包膜侵犯、有脉管侵犯、病理分级Ⅲ+Ⅳ级、TNM分期Ⅲ+Ⅳ期的患者Piwil2高表达组总生存期和无瘤生存期比低表达组短(P均<0.05)。Cox回归分析结果发现,Piwil2表达、TNM分期是影响HCC患者术后无瘤生存期和总生存期的独立影响因素(P<0.05)。结论Piwil2可能参与HCC的发生、发展,其高表达可促使癌细胞增殖与转移,并可影响患者术后生存时间。检测Piwil2在HCC中的表达具有重要的临床价值,并有望成为HCC治疗的新靶标。

Purpose To investigate the expression of Piwil2 in hepatocellular carcinoma(HCC)and to explore its relationship with the occurrence and evolution,clinicopathologic characteristics,and prognosis of HCC.Methods The expression of Piwil2 in 163 HCCs and the corresponding paracancerous tissues was detected with tissue chip technique and immunohistochemiscal PV 6000 method,and statistical analysis was applied.Results The high expression rate of Piwil2 in HCC was 47.85%(78/163),no case of high expression was found in the paracancerous tissues.The difference was statistically significant(χ^2=102.532,P<0.001).The expression of Piwil2 was related to capsule invasion,pathological grade,TNM stage and vascular invasion(all P<0.05).The high expression rate in patients with pathological gradeⅢ+Ⅳand TNM stageⅢ+Ⅳwas higher than that in patients with gradeⅠ+Ⅱ(73.33%vs 26.14%,P<0.001)and stageⅠ+Ⅱ(68%vs 44.2%,P=0.028).The results of Kaplan-Meier prognostic survival analysis showed that the overall survival time and tumor-free survival time of patients with Piwil2 high expression was shorter than that those of patients with low expression of Piwil2(all P<0.05).The total survival time and tumor-free survival time of patients with high expression of Piwil2 were all shorter than those with low expression of Piwil2 in patients with capsule invasion,vascular invasion,gradeⅢ+Ⅳand TNM stageⅢ+Ⅳ(all P<0.05).Cox regression analysis found that the expression of Piwil2 and TNM stage were independent factors affecting the tumor-free survival time and total survival time of HCC patients(P<0.05).Conclusion Piwil2 may be involved in the occurrence and progression of HCC.Its high expression may promote the proliferation and metastasis of HCC,and affect the prognosis of patients.It maybe of great clinical value to detect the expression of Piwil2 in HCC,and may become a new target for treating HCC.