诊断超声波联合超声造影剂提高脑肿瘤化疗疗效的实验研究

Improvement of chemotherapeutic effect of brain tumor by diagnostic ultrasound combined with ultra⁃sound contrast agent

目的探讨微泡介导下诊断超声波辐照兔VX2脑肿瘤提高紫杉醇抗癌效果的可行性。方法建立脑肿瘤模型,并将瘤龄14 d的载瘤兔20只进行随机分组,即对照组(CON);单纯紫杉醇组(PTX);超声+紫杉醇组(US+PTX);超声+微泡+紫杉醇组(PTX+MB+US)。给予各组不同的药物注射及处理方法。各组肿瘤体积在治疗前后均行超声检查并计算体积抑瘤率(TIR)。光镜下观察各组肿瘤细胞凋亡情况,并计算肿瘤细胞凋亡指数。结果诊断超声波辐照兔脑肿瘤US+MB+PTX组TIR高于对照组,差异具有统计学意义(P<0.05)。结论静脉输注超声造影剂的情况下,诊断超声能够显著提高紫杉醇对兔VX2脑肿瘤的化疗效果。

Objective To explore the possibility of improving anticancer effect of paclitaxel in VX2 brain tumor in rabbits by ultrasound irradiation mediated by microbubbles.Methods Brain tumor model was established,and 20 tumor⁃bearing rabbits with tumor age of 14 days were randomly assigned to control group(CON),single taxol group(PTX);ultrasound+paclitaxel group(US+PTX);ultrasound+microbubble+paclitaxel group(PTX+MB+US),with different injections and treatments.The tumor volumes in each group were examined by ultrasound before and after the treatment and the volume inhibition rate(TIR)was calculated.The apoptosis of tumor cells in each group was observed under,and the apoptosis index of tumor cells was calculated.Results The TIR of US+MB+PTX group was higher than that of control group,indicating significant difference(P<0.05).Conclusion In the case of intravenous infusion of ultrasound contrast agent,diagnostic ultrasound can significantly improve the chemotherapy effect of taxol on rabbit VX2 brain tumor.