摘要
为了探究干酪乳杆菌对雏鸡盲肠免疫功能的影响,将450只1日龄SPF健康雏鸡随机分为空白对照组、沙门菌攻毒组、干酪乳杆菌组、预防组、治疗组和预防治疗组,每组5个重复,采用组织学和免疫学方法检测盲肠上皮内淋巴细胞数量及炎性细胞因子的变化。结果显示:饲喂干酪乳杆菌进行预防治疗,可以维护沙门菌感染雏鸡盲肠上皮细胞的完整性,显著提高肠上皮内淋巴细胞的数量,增加抗炎细胞因子IL-4、IL-10的表达量,降低致炎细胞因子IL-17、TNF-α的表达。表明干酪乳杆菌能调节肠道免疫功能,缓解沙门菌诱导的肠道免疫紊乱。
In order to investigate the effect of Lactobacillus casei on the cecal immune function of chicks,450 newborn SPF healthy chicks were randomly divided into blank control group,Lactobacillus casei,Salmonella challenge group,prevention group,treatment group and prevention plus treatment group,and each group was with 5 repeats.Histological and immunological methods were used to observe the changes in the number of cecal intraepithelial lymphocytes and inflammatory cytokines.The results showed that feeding Lactobacillus casei for the prevention and treatment of chicks infected with Salmonella significantly increase the number of intestinal intraepithelial lymphocytes,increased the expression of anti-inflammatory cytokine IL-4 and IL-10,and reduce the expression of inflammatory cytokine IL-17 and TNF-α.
作者
王誉颖
韩德平
闫雪
刘雪连
吴栋
王佳
马云飞
WANG Yu-ying;HAN De-ping;YAN Xue;LIU Xue-lian;WU Dong;WANG Jia;MA Yun-fei(College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;State Key Laboratory of Direct-Fed Microbial Engineering,Beijing DaBeiNong Science and Technology Group Co.,Ltd,Beijing 100192,China)
出处
《中国兽医杂志》
CAS
北大核心
2020年第9期1-4,10,I0001,共6页
Chinese Journal of Veterinary Medicine
基金
国家重点研发计划项目(2018YFD0500606)
国家自然科学基金(31772686,31502025)
北京大北农科技集团股份有限公司饲用微生物工程国家重点实验室开放课题(201205410511191)。
关键词
雏鸡
干酪乳杆菌
上皮内淋巴细胞
细胞因子
chicks
Lactobacillus casei
intraepithelial lymphocytes
cytokines
