摘要
为了从发酵食品中筛选出对牛支原体、牛溶血性曼氏杆菌、牛荚膜A型多杀性巴氏杆菌有拮抗功能的乳酸菌,并进一步对其生物学特性及抑菌特性进行研究。采用牛津杯法抑菌试验筛选得到1株具有优良抑菌活性的鼠李糖乳杆菌,该菌无溶血活性,其抑菌产物具有良好的热稳定性和抗紫外照射活性,抑菌能力随pH降低而增强,经蛋白酶酶解后,抑菌活性有不同程度降低。该株乳酸菌可作为潜在益生菌株防治牛呼吸系统疾病。
The aim of this study was to screen out Lactobacillus with inhibition of Mycoplasma bovis,Mannheimia haemolytica and Pasteurella multocida of bovine capsular type A from fermented food.The biological characteristics and bacteriostatic activity of the compound were also studied.The results showed that a strain of Lactobacillus rhamnosus with excellent bacteriostatic activity was screened by Oxford cup method.The bacterium had no hemolytic activity,the antimicrobial products had good thermal stability and anti-ultraviolet radiation activity.The bacteriostatic activity was increased gradually with the decreasing of pH and the bacteriostatic activity decreased after enzymatic hydrolysis.This study lays a foundation for biological control of bovine respiratory diseases.
作者
党乔
吴信豪
贾博岩
刘磊
马珺杨
刘树明
孔令聪
马红霞
DANG Qiao;WU Xin-hao;JIA Bo-yan;LIU Lei;MA Jun-yang;LIU Shu-ming;KONG Ling-cong;MA Hong-xia(College of Food Science and Enginerring,Jilin Agricultural University,Changchun 130118,China;College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;Key Laboratory of Animal Production and Product Quality and Safety,Ministry of Education,Changchun 130118,China)
出处
《中国兽医杂志》
CAS
北大核心
2020年第9期26-29,33,共5页
Chinese Journal of Veterinary Medicine
基金
国家重点研发计划(2016YFD0501301)
国家自然科学基金(31702293)
吉林省自然科学基金(20190201294JC)。
关键词
乳酸菌
牛支原体
牛溶血性曼氏杆菌
牛荚膜A型多杀性巴氏杆菌
Lactobacillus
Mycoplasma bovis
Mannheimia haemolytica
Pasteurella multocida of bovine capsular type A
