拉曼光谱技术的发展及其在生物医学领域中的应用

Development of Raman spectroscopy and its applications in biomedicine

拉曼光谱技术自发现以来广泛应用于检测固体和液体材料的化学成分,它可利用物质的光谱“指纹”信息,区分各种物质样品、检测不同生理状况的细胞及其中的生物分子,如核酸、蛋白质等.为探索拉曼光谱在生物医学领域的应用前景,在概述拉曼光谱原理的基础上,介绍拉曼光谱技术及其衍生出的其他技术,包括表面增强拉曼散射(SERS)、共振拉曼光谱(RRS)、相干反斯托克斯拉曼光谱(CARS)、受激拉曼光谱(SRS)技术等,最后对拉曼光谱的改进与应用提出展望.

Raman spectroscopy had been widely used to detect the chemical component of solid and liquid materials since its discovery,and had not been used in biomedical research until recent decades.It can use the spectral“fingerprint”of substances to distinguish various samples,detecting cells and biological molecules in different physiological conditions,such as nucleic acids and proteins.This review will outline the principles of Raman spectroscopy,introduce Raman spectroscopy techniques and other derived techniques,including surface enhanced Raman scattering(SERS),resonance Raman spectroscopy(RRS),coherent anti-Stokes Raman Spectroscopy(CARS),stimulated Raman spectroscopy(SRS)technology,etc.,and introduce its application in the field of biomedicine.