150亿孢子/g球孢白僵菌可湿性粉剂的研发及对西花蓟马的防治应用

Research and Development of Wettable Powder of Beauveria bassiana and Its Control and Application to Frankliniella occidentalis

为了研发靶标害虫为西花蓟马的球孢白僵菌杀虫剂,我们首先通过筛选、纯化和复壮获得对西花蓟马高效的真菌菌株;其次,对液-固双相发酵条件和发酵工艺进行优化;然后,改良了产品剂型和配方;最后对产品进行环境和毒性评价。结果显示该产品各项指标均符合国家标准。150亿孢子/g球孢白僵菌可湿性粉剂于2018年在国内获审批登记。喷施1800~3000 g/hm^2对辣椒上西花蓟马的防效均为74%以上。推荐用量为2400 g/hm^2。该产品的研发和获批登记为西花蓟马的生物防治奠定了坚实的基础。

In order to develop an insecticide of Beauveria bassiana against Western Flower Thrips(WFT),Frankliniella occidentalis,firstly,fungal strains with high efficiency to WFT were selected by screening,purification and rejuvenation;secondly,the liquid-solid two-phase fermentation conditions and fermentation process were optimized;thirdly,the product formulation and formula were improved;fourthly,environment and toxicity assessment of the product were evaluated.The results showed that all the indicators of the product were in line with national standards.The 15 billion conidia/g B.bassiana wettable powder was approved and registered in China in 2018.The control efficacies of spraying 1800―3000 g/ha on thrips on pepper are above 74%.The recommended dosage is 2400 g/ha.The development and registration of the product have laid a solid foundation for the biological control of WFT.