东亚小花蝽滞育前后耐寒能力及体内生化物质变化

Changes of Cold Tolerance and Biochemical Substances in Diapause and Post-diapause Orius sauteri

为研究东亚小花蝽滞育前后耐寒能力及与滞育有关的物质变化,以东亚小花蝽雌成虫为供试材料,测定了其滞育、解除滞育和发育3种状态下的过冷却点、低温耐受性及体内耐寒相关物质含量的变化。结果显示,东亚小花蝽雌成虫的过冷却点和结冰点分别为(-9.59±0.61)℃、(-6.28±0.53)℃。滞育与解除滞育的东亚小花蝽的过冷却点、结冰点、体内的含水量显著低于发育个体;滞育东亚小花蝽体内总糖、海藻糖、甘油、脂肪和抗逆性酶含量显著高于发育个体;滞育、解除滞育和发育3种状态的东亚小花蝽体内山梨醇含量差异不显著。本研究表明东亚小花蝽在滞育期间耐寒能力显著提高,这与海藻糖、甘油、脂肪和抗逆性酶含量增加有关。

To explore the changes of cold tolerance and related biochemical substances in diapause and post-diapause Orius sauteri,the super-cooling point(SCP),mortality at low temperature,and contents of cryoprotectant were determined in O.sauteri female adults under normal development,in diapause and at post-diapause.The SCP and FP(freezing point)of female adults were(-9.59±0.61)℃and(-6.28±0.53)℃,respectively.The SCP,FP and content of free water in diapausing and post-diapause nymphs were significantly lower than those in normal development individuals.However,total carbohydrate,trehalose,glycerin,total-fat and activities of stress-tolerant enzymes in diapausing nymphs were significantly higher than those in the normal development ones.The content of sorbitol in diapause insects was not significantly different from those in the other treatments.The results indicate that the cold tolerance of diapause O.sauteri is improved,which is probably related with the increase of the content of total carbohydrate,trehalose,glycerin,total-fat and activities of stress-tolerant enzymes.