ABA对拟南芥AtHsfA1a在冷胁迫响应中生理指标的影响

Effects of ABA on Physiological Indexes of Arabidopsir AtHsfA1a in Response to Cold Stress

为从生理水平探讨低温下脱落酸(ABA)与拟南芥热激因子AtHsfA1a的关系,以拟南芥热激因子AtHsfA1a基因T-DNA插入突变型(MT)和野生型(WT)拟南芥为实验材料,研究外源ABA对AtHsfA1a不同基因型拟南芥在低温下的生理指标的影响.结果表明,低温可使幼苗中MDA和H2O2增加,AtHsfA1a基因突变型增加较明显,加入外源ABA后MDA和H2O2有所降低,AtHsfA1a基因突变型降低较少.而抗氧化酶变化趋势正好相反,突变型拟南芥的SOD、CAT、GR和APX活性在低温中比野生型升高小,ABA对冷胁迫中突变型拟南芥抗氧化酶SOD、CAT、GR和APX活性影响较小.说明外源ABA在AtHsfA1a提高抗冷性过程中有调控作用.

In order to study the relationship between abscisic acid(ABA) and Arabidopsis heat shock factor AtHsfA1 a at low temperature, Arabidopsis heat shock factor AtHsfA1a gene Mutant of T-DNA insertion(MT)and wild type(WT)of Arabidopsis were used as experimental materials to study the effect of Exogenous ABA on the physiological indexes of different AtHsfA1a genotypes of Arabidopsis seedlings at low temperature. The results showed that the content of MDA and H202 in the Arabidopsismutant increased significantly. The content of MDA and H2O2 decreased after the addition of Exogenous ABA, while that of MDA and H2O2 decreased less in AtHsfA1a gene mutation, but the change trend of antioxidant enzymes was the opposite. The SOD, CAT, GR and APX of Arabidopsis mutant increased less;ABA had little effect on the activity of SOD, CAT, GR and APX of Arabidopsis mutant under cold stress. It is suggested that exogenous ABA can regulate on the process of improving the cold resistance of AtHsfA1 a.