无花果多糖分离纯化工艺及抗氧化性能研究

Study on Separation and Purification Process and Antioxidant Properties of Fig Polysaccharides

探讨无花果多糖分离纯化工艺及抗氧化性能,以无花果为试材,通过选用不同树脂去除无花果多糖中的色素,结果表明使用AB-8树脂效果最好;利用3%三氯乙酸法去除蛋白质,选用0.03 mg/mL的NaCl溶液洗脱纯化,使用AB-8柱层析色谱图在管数为10~20,30~40时出现脱峰,分离纯化完成。通过测定清除超氧阴离子自由基、羟基自由基和还原能力,评价其抗氧化性能。结果表明,当浓度为2500μg/mL时,无花果超氧阴离子清除效率和羟基自由基清除效率都达到最高,分别为75%和38%。在1000μg/mL浓度下,还原量最大,无花果多糖分离纯化后具有较高的抗氧化性能,该研究结果可为无花果产业生产提供技术支持。

To explore the separation and purification process and antioxidant properties of polysaccharides from figs.Different resins are used to remove pigments from fig polysaccharides,and the results show that the effect of AB-8 resin is the best;to remove proteins by 3%trichloroacetic acid method,using 0.03 mg/mL NaCl solution for elution and purification,and AB-8 column chromatogram shows that the de-peaking phenomenon occours when the tubes'number is 10~20,30~40 and the separation and purification are completed.By measuring the scavenging capacity of superoxide anion free radicals,hydroxyl free radicals and reducing ability,the antioxidant properties are evaluated.The results show that when the concentration is 2500μg/mL,the superoxide anion scavenging efficiency and hydroxyl radical scavenging efficiency of figs reach the highest,which is 75%and 38%respectively.At a concentration of 1000μg/mL,the reduction amount is the largest,and the polysaccharides from figs have high antioxidant properties after separation and purification.The research results can provide technical support for the production of fig industry.