SNF5在膀胱癌中的表达水平及其临床意义

Expression and clinical significance of SNF5 in bladder cancer

目的探讨SNF5在膀胱癌中的表达水平及临床意义。方法分别利用癌症基因组图谱数据库(the Cancer Genome Atlas,TCGA)分析RNA-seq数据、qPCR检测膀胱癌细胞系,研究膀胱癌中SNF5表达水平,并结合TCGA临床样本信息行Kaplan-Meier生存分析研究SNF5对预后的影响。收集2012年1月至2018年12月我科收治的157例膀胱癌患者肿瘤组织及临床信息,免疫组化法检测膀胱癌组织中SNF5蛋白水平,分析其与临床病理特征关系。结果在mRNA水平,SNF5在膀胱癌组织中和膀胱癌细胞系中均高于正常膀胱组织,在不同mRNA膀胱癌亚型和膀胱癌细胞系中表达也存在差异。生存分析中提示SNF5 mRNA低表达组患者总体生存时间明显缩短(P<0.01),而Cox分析进一步提示SNF5是膀胱癌独立预后因素。在蛋白水平,回顾性研究发现SNF5表达水平与肌层浸润(P<0.01)、淋巴结阳性(P<0.01)密切相关。结论SNF5在膀胱癌中表达上调,与膀胱癌发生、发展及预后密切相关,具有作为潜在治疗靶点的潜能。

Objective To explore the expression and clinical significance of SNF5 in bladder cancer.Methods Based on the data from the Cancer Genome Atlas(TCGA)database,SNF5 mRNA level from 408 cases of bladder cancer tissues and 19 cases of normal bladder tissues were retrieved and analyzed with clinical data.Kaplan-Meier analysis was employed to study the relationship between SNF5 level and survival rate in these bladder cancer patients.qPCR was performed to determine the expression level of SNF5 in different bladder cancer cell lines(5637,T24,RT4,UM-UC-3,TCCSUP,J82)and normal human urothelial cell line SV-HUC-1.What’s more,the protein expression of SNF5 in clinically collected bladder cancer samples(from 157 patients admitted in our hospital from January 2012 to December 2018)was detected by immunohistochemical(IHC)assay,and its association with clinicopathological features was analyzed with Cox regression analysis.Results The mRNA expression of SNF5 was higher in both bladder cancer tissues and cell lines than normal bladder tissues and cells,and the expression variations were significant among different subtypes of bladder cancer and cancer cell lines.The results of Kaplan-Meier analysis indicated that the patients with lower SNF5 mRNA level had worse prognosis than those with higher expression(P<0.01).Cox regression analysis showed SNF5 was an independent prognostic factor for bladder cancer.The protein level of SNF5 was significantly correlated with myometrial infiltration(P<0.01)and lymph node metastasis(P<0.01).Conclusion SNF5 is upregulated in bladder cancer,and closely associated with its incidence,progression and prognosis.It may be used as a promising therapeutic target in treatment for bladder cancer.