北美鹅掌楸淹水胁迫转录因子的鉴定及表达分析

Identification and Expression Analysis of Transcription Factors in Liriodendron tulipifera under Flooding Stress

北美鹅掌楸是优良的绿化和用材树种,但不耐水涝的特性严重影响其在季节性淹水地区推广种植。利用新一代Illumina HiSeq^TM PE100高通量转录组测序技术,本研究对1个耐淹无性系和1个淹水敏感无性系淹水胁迫4 d下的叶片的转录因子基因进行了鉴定。结果表明,转录组测序共获得214 161个Unigene序列,鉴定出77个转录因子家族。不同无性系淹水胁迫后转录因子表达存在差异。在耐淹无性系中,检测到淹水后差异表达基因上调基因数量2 015个,转录因子103个,差异基因下调表达的数量1 793个,转录因子基因91个;而在淹水敏感无性系检测到的10 389个差异表达基因中,上调表达的转录因子217个,下调表达的转录因子191个。MYB、Zinc finger、NAC、Orphans、WRKY、Homebox domain、AP2 domain、bHLH、AUX/IAA family等转录因子家族在北美鹅掌楸淹水后差异表达基因数量最多。本研究初步分析了淹水环境下不同北美鹅掌楸无性系转录因子的特征表达谱,为深入研究转录因子对植物淹水胁迫的响应、调控机制提供了参考。

Liriodendron tulipifera is an excellent greening tree species and timber, but its poor flooding resistance seriously affects its application in seasonal waterlogged areas. In this study, the identification and gene expression pattern of the transcription factors of the leaves of one flooding-tolerant clone and one flooding-sensitive clones were analysed by the high-through put transcriptome sequencing technology of Illumina Hiseq^TM PE100. The results showed that 214 161 Unigene sequences were obtained and 77 families of transcription factor were identified. The expression models of the transcription factors in different clones were different. A total of 2015 genes were up regulated in flood resistant clones, in which, 103 transcription factor genes were identified, and 91 transcription factor genes were down regulated in 793 down regulated genes. There are 217 up-regulated and 191 downregulated transcription factors in 10 389 DEGs of flood sensitive clones. MYB, Zinc finger, NAC, Orphans, WRKY,Homebox domain, AP2 domain, b HLH and AUX/IAA family were the most differentially expressed transcription factors under flooding. In this study, we analyzed the expression profiles of transcription factors in different clones of Liriodendron tulipifera under flooding environment, which provided a reference for further study on the regulatory mechanism of transcription factors responding to flooding stress.