玉米SDG108基因的克隆及遗传转化拟南芥

Cloning of SDG108 Gene and Its Transformation in Arabidopsis thaliana

为研究玉米组蛋白赖氨酸甲基化基因SDG108的功能,本实验克隆了全长为6 789 bp的玉米SDG108基因,该基因编码2 262个氨基酸,并构建重组质粒载体,采用浸花法将重组质粒基因转入到拟南芥野生型内,通过除草剂、PCR和试纸条对抗性植株进行鉴定,共筛选出了7株阳性拟南芥植株。对阳性拟南芥植株后代进行表型鉴定及基因表达量鉴定。结果表明,与野生型相比转基因拟南芥开花时间提前,莲座叶直径增大,叶片长度、宽度及叶柄长度均有所增加;叶片数量减小,一级分枝数无明显差异。基因表达方面,转化株的FLC基因表达量下降,SOC1基因表达量上升。研究结果揭示了SDG108基因对拟南芥在开花及莲座叶片表型方面的影响。

To study the functions of SDG108,a histone lysine methylation gene in maize a 6789 bp maize SDG108 gene was cloned in this experiment,which encodes 2262 amino acids,and a recombinant plasmid vector was constructed.The recombinant plasmid gene was transferred into Arabidopsis thaliana wild-type by floral-dip-ping method.We identified 7 transformed Arabidopsis thaliana plants by herbicides,PCR and test strip.Identifica-ting phenotype and gene expression of the transformed Arabidopsis thaliana showed that the flowering stage of transgenic plants was earlier than wild-type,rosette diameter,leaf length,leaf width and petiole length increased and the number of leaves decreased.There was no significant difference in the number of primary branches.In terms of gene expression,FLC in the transformed plant decreased while SOC1 increased,compared with the wild type.The study results revealed the influence of SDG 108 on the flowering time and rosette leaves of A.thaliana.