摘要
脉冲电场可以改变细胞信号转导、促进伤口愈合和细胞生长、诱导外源分子跨细胞膜输运等,广泛应用于生物科技领域。以囊泡作为细胞膜系统模型,简要介绍了电穿孔技术的发展历程,主要综述了脉冲电场导致囊泡成孔的作用机制,并展望了未来的发展趋势。
Pulsed electric field(PEF)can change cell signal transduction,promote wound healing and cell growth,and induce the transport of exogenous molecules across cell membranes,which has been widely used in the field of biotechnology.Using vesicle as the model of cell membrane system,we briefly introduce the development of electroporation technology,mainly review the pores formation mechanism of vesicles induced by PEF,and prospect the future development trend.
作者
石铭芸
蒋中英
SHI Mingyun;JIANG Zhongying(Laboratory of Micro-Nano Electro Biosensors and Bionic Devices,School of Electronics and Information Engineering,Yili Normal University,Yining 835000,China)
出处
《化学与生物工程》
CAS
2021年第1期5-10,共6页
Chemistry & Bioengineering
基金
新疆自治区研究生科研创新项目(XJ2020G302)
国家自然科学基金项目(21764015,11847610)。
关键词
电穿孔
巨型单层囊泡(GUVs)
脉冲电场
成孔作用机制
electroporation
giant unilamellar vesicles(GUVs)
pulsed electric field
pore formation mechanism
