摘要
以自制的羧甲基壳聚糖-油酸聚合物(CMCS-OA)为载体,采用透析法对盐酸阿霉素(DOX)进行包载,载药聚合物(DOX-CMCS-OA)的载药量和包封率分别达到30.28%和65.83%。体外释药实验表明,DOX-CMCS-OA在pH值为5.0和7.4下1660 min时累积释药率分别为262.29%和232.64%,偏酸性环境下释药效果更好,具有一定的pH值敏感性。细胞毒性实验表明,DOX-CMCS-OA对SGC-7901胃癌细胞和SK-MES-1肺癌细胞均具有较好的抑制作用且比DOX的毒性更低。细胞摄取实验表明,DOX-CMCS-OA具有较好的药物控释性能。CMCS-OA对DOX的包载能力较好,具有一定的缓释、长效的抗肿瘤作用。
By using self-made carboxymethyl chitosan-oleic acid(CMCS-OA)polymer as a carrier,Doxorubicin hydrochloride(DOX)was loaded by a dialysis method.The drug loading and encapsulation rate of drug-loading polymer DOX-CMCS-OA can reach 30.28%and 65.83%,respectively.The in vitro drug release experiments show that the cumulative drug release rate of the DOX-CMCS-OA are 262.29%and 232.64%at the pH values of 5.0 and 7.4 for 1660 min,respectively,indicating that the drug release effect is better under an acidic environment,and the DOX-CMCS-OA has a certain pH value sensitivity.The cytotoxicity experiments show that the DOX-CMCS-OA has a better inhibitory effect on SGC-7901 gastric cancer cells and SK-MES-1 lung cancer cells,and has less toxicity than DOX.The cellular uptake experiments show that the DOX-CMCS-OA has a better controlled release property.In conclusion,the CMCS-OA has a good encapsulation ability for DOX and has a certain sustained-release and long-term antitumor effect.
作者
尤静
张文涛
苗延青
张雪娇
梁玲玲
YOU Jing;ZHANG Wentao;MIAO Yanqing;ZHANG Xuejiao;LIANG Lingling(Department of Pharmacy,Xi′an Medical University,Xi′an 710021,China)
出处
《化学与生物工程》
CAS
2021年第1期22-25,33,共5页
Chemistry & Bioengineering
基金
陕西省教育厅专项科研计划项目(18JK0665)
陕西省科技厅自然科学项目(2019JQ-887)
西安医学院校级项目(2018PT62)。
关键词
羧甲基壳聚糖-油酸聚合物
载药
释药
抗肿瘤
carboxymethyl chitosan-oleic acid polymer
drug loading
drug release
antitumor