摘要
基于指纹图谱和网络药理学进行竹茹汤全过程质量控制和功效关联物质预测分析。建立药材、饮片、竹茹汤物质基准的指纹图谱,标定了25个共有峰,指认了3'-羟基葛根素、葛根素、3'-甲氧基葛根素、葛根芹菜糖苷、大豆苷、甘草苷、大豆苷元、甘草酸铵、6-姜辣素9个共有色谱峰,相似度均>0.95。主要药效物质群可以从药材-饮片-物质基准逐级较为完整的传递,且归属关系清晰。基于可测性和可追溯性进行活性成分的筛选,采用网络药理学的方法,对筛选的9个化学成分进行"成分-靶点-通路"网络关系的构建与分析,富集的通路中含能量代谢、酒精中毒、平滑肌的收缩与舒张相关通路;结果表明竹茹汤的这9个有效成分可能通过作用与这些信号通路达到清热解酒、和胃止呕的作用。基于以上9个成分具有传递性和溯源性,且与竹茹汤功效属性密切相关,可作为潜在功效关联物质,为全面控制竹茹汤的质量提供依据。
Based on fingerprint and network pharmacology,the whole process quality control of Zhuru Decoction was conducted and efficacy-related substances were predicted.The fingerprints of raw materials,decoction pieces and Zhuru Decoction were established,and 25 common peaks were identified,including 9 common chromatographic peaks of 3′-hydroxy puerarin,puerarin,3′-methoxy puerarin,puerarin,aperioside,daidzin,daidzein,liquiritin,glycyrrhizic acid and 6-gingerol,with similarity all greater than 0.95.The main groups of pharmacodynamic substances can be transferred from raw materials,decoction pieces to Zhuru Decoction step by step,with a clear affiliation relationship.Based on the testability and traceability,the active ingredients were screened,and the network relationship of"component-target-pathway"was constructed and analyzed for the nine chemical components screened by network pharmacology.The enriched pathways included energy metabolism,alcoholism,and smooth muscle contraction and relaxation-related pathways.The nine active components of Zhuru Decoction may achieve the effects of clearing heat,alleviating a hangover,harmonizing stomach and stopping vomiting through these signaling pathways.Based on transitive and traceable properties of the above 9 components as well as their close relationship to the efficacy of Zhuru Decoction,these 9 components can be identified as potential efficacy-related substances and provide basis for the overall quality control of Zhuru Decoction.
作者
许金国
黄紫炎
沈钱能
李林
王巧晗
王璐
陆兔林
毛春芹
XU Jin-guo;HUANG Zi-yan;SHEN Qian-neng;LI Lin;WANG Qiao-han;WANG Lu;LU Tu-lin;MAO Chun-qin(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2020年第23期5599-5606,共8页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2018YFC1707000)。
关键词
经典名方
竹茹汤
指纹图谱
网络药理学
功效关联物质
质量控制
classical prescription
Zhuru Decoction
fingerprint chromatography
network pharmacology
efficacy related substance
quality control