经典名方黄芪桂枝五物汤标准煎液化学成分的UPLC-Q-TOF-MS分析

UPLC-Q-TOF-MS analysis on chemical constituents of classical prescription Huangqi Guizhi Wuwu Tang standard decoction

采用UPLC-Q-TOF-MS技术解析经典名方黄芪桂枝五物汤标准煎液的化学成分。以Acquity HSS T3(2.1 mm×100 mm,1.8μm)为色谱柱,0.1%甲酸水-0.1%甲酸乙腈为流动相进行梯度洗脱,流速0.4 mL·min^-1,柱温40℃。在正、负离子模式下采集黄芪桂枝五物汤标准煎液的质谱数据。通过Masslynx 4.1软件结合SciFinder数据库、对照品比对、质谱数据分析及参考相关文献对黄芪桂枝五物汤标准煎液的化学成分分析和鉴定。共分析和鉴定出110个化合物,包括33个黄酮类化合物、14个单萜苷类化合物、8个三萜类化合物、8个姜辣素类化合物、17个苯丙素类化合物、12个有机酸类化合物、7个氨基酸类化合物以及11个其他类化合物。该研究结果为黄芪桂枝五物汤标准煎液的进一步药效物质基础研究及质量控制的建立提供了实验依据。

UPLC-Q-TOF-MS technology was used to analyze the chemical constituents from classical prescription Huangqi Guizhi Wuwu Tang standard decoction.Acquity HSS T3 column(2.1 mm×100 mm,1.8μm)was used as the chromatographic column,with 0.1% formic acid solution-0.1% formic acid acetonitrile as the mobile phase for gradient elution.The volume flow rate was 0.4 mL·min^-1 and the column temperature was 40℃.Mass spectrometry data of Huangqi Guizhi Wuwu Tang standard decoction were collected in positive and negative ion modes.The chemical constituents from classical prescription Huangqi Guizhi Wuwu Tang standard decoction were analyzed and identified by Masslynx 4.1 software combined with SciFinder database,comparison with reference mate-rials,mass spectrometry data analysis and reference to relevant literature.A total of 110 compounds were analyzed and identified,including 33 flavonoids,14 monoterpene glycosides,8 triterpenoids,8 gingerols,17 phenylpropanoids,12 organic acids,7 amino acids and 11 other compounds.The results of this study provide an experimental basis for the further research on the substance basis and quality control of Huangqi Guizhi Wuwu Tang standard decoction.