丹参产地加工方法对急性心肌缺血大鼠模型的影响研究

Study on effects of different habitat processing methods of Salviae Miltiorrhizae Radix et Rhizoma in rats with acute myocardial ischemia

研究丹参产地加工方法对垂体后叶素诱发大鼠急性心肌缺血的影响。该研究采用尾静脉注射垂体后叶素诱导大鼠急性心肌缺血,记录心电图指标心率、ΔST变化,测定大鼠血清中肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)、超氧化歧化酶(SOD)、丙二醛(MDA)的含量,综合评分法评价6种产地加工方法加工的丹参药材对急性心肌缺血大鼠血清生化指标的影响。心电图结果显示:烘干法加工的丹参药材对缺血后心电图心率、ΔST的改善效果较好,其余各组均有一定保护作用。缺血后血清中各组生化指标测定结果显示:烘法减重发汗后烘干法高剂量组、阴干法高剂量组、阴干法低剂量组对CK-MB活性降低最为显著;阴干法高剂量组、阴干法低剂量组对LDH活性降低最为显著;晒干法低剂量组、晒法减重发汗后晒干法低剂量组、烘干法低剂量组对SOD活性升高最为显著;晒干法低剂量组对MDA活性降低最为显著。综合评分结果显示,阴干法高剂量组评分最高,其余组均较模型组高。烘干法对急性心肌缺血后心电图改善效果较好,阴干法对急性心肌缺血后血清中心肌酶改善作用较好,其余加工方法对心肌缺血具有一定的保护作用。通过药效学对6种加工方法进行评价,阴干法、烘干法加工的丹参药材药效较好。

To investigate the effects of different habitat processing methods of Salviae Miltiorrhizae Radix et Rhizoma on acute myocardial ischemia induced by pituitrin in rats.In this experiment,the tail vein injection of pituitrin was used to induce acute myocardial ischemia in rats.Electrocardiograph(ECG)heart rate and ΔST changes were recorded,and the levels of creatine kinase isoenzyme(CK-MB),lactate dehydrogenase(LDH),superoxide dismutase(SOD)and malondialdehyde(MDA)in serum of rats were detected to comprehensively evaluate the effects of six processing methods of Salviae Miltiorrhizae Radix et Rhizoma on serum biochemical indexes of rats with acute myocardial injury.The ECG results showed that the Salviae Miltiorrhizae Radix et Rhizoma dried in a drying oven had a good effect on the improvement of heart rate andΔST of electrocardiogram after ischemia,and all the other groups had some protective effects to different degrees.The results of biochemical indexes in serum of each group after ischemia showed that the activity of CK-MB decreased most significantly in Salviae Miltiorrhizae Radix et Rhizoma high-dose group with drying in a drying oven after sweating and losing weight in a drying oven,high-dose group with drying in the shade and low-dose group with drying in the shade.The activity of LDH decreased most significantly in Salviae Miltiorrhizae Radix et Rhizoma high-dose group with drying in the shade and low-dose group of drying in the shade.The activity of SOD increased most significantly in Salviae Miltiorrhizae Radix et Rhizoma low-dose group with drying in sun,low-dose group with drying in sun after sweating and losing weight in sun,and low-dose group with drying in a drying oven.The activity of MDA decreased most significantly in Salviae Miltiorrhizae Radix et Rhizoma low-dose group with drying in sun.The comprehensive scoring results showed that the highest score was obtained in Salviae Miltiorrhizae Radix et Rhizoma high-dose group with drying in the shade while the scores of other treatment groups were higher than that of the model group.It could be seen that the Salviae Miltiorrhizae Radix et Rhizoma dried in a drying oven had a good improvement effect on electrocardiograph indexes after acute myocardial injury,the Salviae Miltiorrhizae Radix et Rhizoma dried in the shade had a good improvement effect on serum myocardial enzymes after acute myocardial injury,and the other processing methods had a certain protective effect on myocardial injury.The six processing methods evaluated by pharmacodynamics showed that the Salviae Miltiorrhizae Radix et Rhizoma dried in the shade and dried in a drying oven had good efficacy.