基于网络药理学的菊苣抗高脂血症作用机制研究

Research on anti-hyperlipidemia mechanisms of Cichorii Herba based on network pharmacology

目的采用网络药理学方法,揭示菊苣抗高脂血症的活性成分、潜在药物靶点和作用机制。方法通过公共数据库中获取菊苣的活性成分、潜在药物靶点以及高脂血症的相关基因,并结合相关软件进行网络药理学分析,包括蛋白质-蛋白质相互作用(PPI)、基因本体(GO)分析和京都基因和基因组百科全书(KEGG)分析。结果从TCMSP数据库共筛选菊苣活性成分12个,包括β-谷甾醇、木犀草素等核心成分,涉及相关基因靶点156个。PPI网络涉及56个潜在药物靶点,Degree值排名前5的核心靶点分别是AKT1、IL6、TP53、TNF、VGEFA。GO功能分析共获得269个条目(P<0.05),KEGG通路富集得到71条信号通路(P<0.05),包括VEGF信号通路、脂肪细胞因子信号通路、细胞凋亡等通路。结论菊苣抗高脂血症是多成分、多靶点、多通路的综合效应,其机制可能与参与脂质合成、炎症反应、细胞凋亡等过程相关。

Objective To reveal the active components,potential targets and action mechanisms of Cichorii Herba against hyperlipidemia by network pharmacology.Methods The biologically active ingredients and potential drug targets of Cichorii Herba and hyperlipidemia-related genes were obtained from public databases,and corresponding network pharmacological analysis were performed with software,including protein-protein interaction(PPI),gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG).Results A total of 12 active ingredients of Cichorii Herba were screened from the TCMSP database,including core drugsβ-sitosterol,luteolin,and 156 related gene targets.The PPI network involves 56 potential drug targets,and the top 5 core targets with Degree values are AKT1,IL6,TP53,TNF,and VGEFA.GO functional analysis obtained a total of 269 items(P<0.05),and KEGG pathway enrichment resulted in 71 signal pathways(P<0.05),including VEGF signal pathway,adipocyte factor signal pathway,apoptosis and other pathways.Conclusion The anti-hyperlipidemia of Cichorii Herba is a comprehensive effect of multiple components,multiple targets and multiple pathways,and its mechanisms may be related to the processes involved in lipid synthesis,inflammation,and cell apoptosis.