肝细胞癌组织中miR-548c-3p、TRIM59的表达变化及其意义

Expression change and significance of miR-548c-3p and TRIM59 in hepatocellular carcinoma tissues

目的分析肝细胞癌(HCC)组织中微小RNA 548c-3p(miR-548c-3p)、三基序蛋白59(TRIM59)mRNA的表达及意义。方法运用实时荧光定量PCR检测114例HCC组织及癌旁组织中的miR-548c-3p、TRIM59 mRNA;分析miR-548c-3p、TRIM59 mRNA表达与患者临床病理特征的关系;随访5年,采用Kaplan-Meier法分析不同miR-548c-3p、TRIM59 mRNA表达患者的生存差异。结果HCC组织中miR-548c-3p的相对表达量低于癌旁组织,而TRIM59 mRNA表达高于癌旁组织(P均<0.05)。HCC组织中miR-548c-3p、TRIM59 mRNA表达与TNM分期、淋巴结转移和分化程度有关(P均<0.05)。HCC组织中miR-548c-3p与TRIM59 mRNA表达呈负相关(r=-0.735,P<0.05)。Kaplan-Meier生存分析结果显示,miR-548c-3p低表达者5年总体生存率(OS)低于miR-548c-3p高表达者;TRIM59 mRNA高表达者OS低于TRIM59低表达者(P均<0.05)。结论HCC组织中miR-548c-3p表达降低,TRIM59 mRNA表达升高,两者与TNM分期、淋巴结转移、分化程度及预后有关,有可能成为新的HCC诊治及预后的分子标志物。

Objective To analyze the expression changes of microRNA 548c-3p(miR-548c-3p)and tripartite motif protein 59 mRNA(TRIM59 mRNA)in the hepatocellular carcinoma(HCC)tissues and their significance.Methods The expression levels of miR-548c-3p and TRIM59 mRNA in 114 HCC tissues and adjacent tissues were detected by fluo⁃rescence real-time quantitative PCR(qRT-PCR);the relationship between miR-548c-3p and TRIM59 mRNA expression and clinicopathological characteristics was analyzed;they were followed up for 5 years,and Kaplan-Meier survival analysis was used to analyze the survival difference between patients with different miR-548c-3p and TRIM59 mRNA expression.Results The relative expression of miR-548c-3p in HCC tissues was lower than that in the adjacent tissues,while the ex⁃pression of TRIM59 mRNA was higher than that in the adjacent tissues(both P<0.05).The expression levels of miR-548c-3p and TRIM59 mRNA in HCC tissues were related to TNM stage,lymph node metastasis,and degree of differentia⁃tion(all P<0.05).There was a negative correlation between miR-548c-3p and TRIM59 mRNA expression in HCC tissues(r=-0.735,P<0.05).Kaplan-Meier survival analysis showed that the 5-year overall survival(OS)of patients with low ex⁃pression of miR-548c-3p was lower than that of patients with high expression of miR-548c-3p;the OS of patients with high expression of TRIM59 mRNA was lower than that of patients with low TRIM59 mRNA expression(all P<0.05).Conclu⁃sion The expression of miR-548c-3p decreases,the expression of TRIM59 mRNA increases in HCC tissues,and both of them are related to TNM stage,lymph node metastasis,differentiated degree and prognosis,which may become new molec⁃ular markers for the diagnosis,treatment,and prognosis of HCC.