虑烦汤对大鼠缺血性卒中后焦虑行为影响的研究

Effect of Lvfan Decoction on anxiety behavior after ischemic stroke in rats

[目的]探讨虑烦汤不同剂量对大鼠缺血性卒中后焦虑行为影响。[方法]选取SPF级雄性健康SD成年大鼠,采用大脑中动脉局灶性脑缺血复合空瓶刺激大鼠焦虑模型。将制备成功的模型大鼠随机分为模型组,盐酸丁螺环酮组(3 mg/kg),虑烦汤低剂量组(1.6 g/kg)和高剂量组(3.2 g/kg),同时设置空白组。连续灌胃给药14 d,在末次给药1 h后,通过高架十字迷宫,旷场实验、明暗箱实验以及Morris水迷宫等行为学实验,观察虑烦汤不同剂量对大鼠焦虑行为的干预效果。[结果]高架十字迷宫实验提示,模型组大鼠进入开放臂时间比和次数比均减小,与空白组比较差异具有统计学意义(P<0.01)。虑烦汤能明显增加大鼠在旷场运动的总路程和进入中央区时间,有增加大鼠进入开放臂时间比和次数比的趋势。旷场实验、明暗箱实验以及Morris水迷宫等行为学实验均提示,复合模型大鼠出现了自主活动减少,紧张恐惧等焦虑的行为学现象,虑烦汤干预后,大鼠自主活动和探索行为有所增加,与前期临床研究相符合。[结论]虑烦汤有一定的抗焦虑作用,高剂量虑烦汤剂效果更显著。

[Objective]To investigate the effect of different doses of Lvfan Decoction on anxiety behavior after ischemic stroke in rats.[Methods]SPF male healthy SD adult rats were used the rat anxiety model of middle cerebral artery focal cerebral ischemia combined with empty bottle stimulation.The successfully prepared model rats were randomly divided into model group,buspirone hydrochloride group(3 mg/kg),Lvfan Decoction low(1.6 g/kg)and high-dose group(3.2 g/kg),and blank control group.Continuous intragastric administration for 14 days,and 1 hour after the last administration,observe the intervention effect of different doses of Lvfan Decoction on anxiety behavior of rats through behavioral experiments such as elevated cross maze,open field experiment,light and dark box test and Morris water maze.[Results]The elevated cross maze experiment showed that the time ratio and frequency ratio of rats entering the open arm in the model group were reduced,and the difference was significant statistically compared with the blank control group(P<0.01).Lvfan Decoction can obviously increase the total distance of rats in open field and the time of entering the central area,and has a tendency to increase the time ratio and frequency ratio of rats entering the open arm.Behavioral experiments,such as open field experiment,bright black box test and Morris water maze,all suggest that the compound model rats have anxious behavioral phenomena such as decreased autonomous activity and nervous fear.After the intervention of Lvfan Decoction,the autonomous activity and exploratory behavior of rats have increased,which is consistent with the previous clinical studies.[Conclusion]Lvfan Decoction has certain anti-anxiety effects,and the effect of high-dose Lvfan Decoction is more remarkable.