黏蛋白16在神经胶质瘤组织中表达及意义

Expression and significance of mucin 16 in glioma

目的检测黏蛋白16(MUC16)在神经胶质瘤组织中的表达情况,探讨下调MUC16基因表达对U87细胞增殖、迁移和侵袭力的影响。方法选取2013年5月—2019年5月在焦作市人民医院行手术治疗的神经胶质瘤患者86例及因颅脑外伤行手术治疗的患者45例。免疫组织化学法检测神经胶质瘤和正常脑组织中MUC16蛋白表达水平,培养U87细胞并分为MUC16干扰组、阴性对照组和空白组,分别利用实时荧光定量PCR、MTT法、划痕实验和Transwell法检测U87细胞中MUC16表达情况,以及各组细胞增殖、迁移和侵袭能力。结果MUC16蛋白在神经胶质瘤患者中阳性表达率73.26%,正常脑组织为26.67%,两者比较差异有统计学意义(P<0.001);MUC16蛋白在不同WHO分级阳性表达率比较,差异有统计学意义(P<0.05);MUC16干扰组MUC16 mRNA相对表达量,24、48、72和96 h时吸光度(A)值、24和48 h时划痕愈合率、侵袭细胞数均低于阴性对照组和空白组,差异有统计学意义(P<0.05)。结论神经胶质瘤组织中MUC16呈高表达,且与WHO分级呈正相关,下调U87细胞中MUC16基因表达可抑制细胞增殖、迁移和侵袭能力。

Objective To investigate the expression of mucin 16(MUC16)in glioma tissues,and the effect of down-regulation of MUC16 gene expression on proliferation,migration and invasion of human glioma U87 cells.Methods A total of 86 cases of patients with glioma who underwent surgery treatment in our hospital were selected from May 2013 to May 2019.In the same period,45 patients undergoing surgical treatment for craniocerebral trauma in our hospital were selected.The expressions of MUC16 proteins in glioma and normal brain tissues were detected by using immunohistochemistry.The U87 cells were cultured and divided into MUC16 interference group,negative control group and blank group.Real-time fluorescent quantitative PCR,MTT,scratch experiment and Transwell method were used to detect the expression of MUC16 in cells,and cell proliferation,migration and invasion ability.Results The positive expression rate of MUC16 protein in glioma patients was 73.26%,which was higher than 26.67%in the normal brain tissues,the difference was statistically significant(χ2=26.200,P<0.001).The difference of the positive expression rate of MUC16 protein between different WHO grades were statistically significant(P<0.05).The relative expression level of MUC16 mRNA in the cells,and theabsorbance A values at 24,48,72 and 96 h,the scratchhealing rates at 24 and 48 h,the number of invasive cells in the MUC16 interference group were lower than those in the negative control group and the blank group,and the differences were statistically significant(P<0.05).Conclusions MUC16 is highly expressed in glioma tissues and is positively correlated with WHO grade.Down-regulation of MUC16 gene expression in U87 cells can inhibit cell proliferation,migration and invasion ability.