感染16型蓝舌病病毒后绵羊部分细胞因子消长特点研究

Study on the Growth and Decline Characteristics of Some Cytokines in Sheep after Infected with Bluetongue Virus Type 16

本试验旨在探明绵羊感染16型蓝舌病病毒(Bluetongue virus type 16,BTV16)后细胞因子IFN-γ、IL-2、IL-4和IL-10的消长特点。用实时荧光定量PCR检测方法对感染BTV16后3只绵羊上述4种因子mRNA进行检测,同时设立阴性对照绵羊,并以0 d mRNA为基准,计算mRNA的相对表达量,同时检测病毒抗体效价、测量绵羊体温。结果显示,接种BTV16的3只绵羊均不同程度产生抗体和体温症状,4种细胞因子的mRNA在接种病毒2~4 d内均出现显著上升,其中IFN-γ峰值在2.58~27.84倍之间,IL-2峰值在5.24~17.19倍之间,IL-4峰值在2.16~3.43倍之间,IL-10峰值在15.78~48.77倍之间,个体上升幅度存在显著差异,4种细胞因子均在高水平持续6 d左右后逐渐下降。对照绵羊上述参数在正常范围内波动。本研究阐明了接种BTV16后绵羊细胞因子IFN-γ、IL-2、IL-4、IL-10在转录水平上的消长特点,为进一步深入开展BTV感染特征、宿主机体免疫机制研究提供参考。

The purpose of this experiment was to investigate the growth and decline characteristics of IFN-γ,IL-2,IL-4,IL-10 of sheep after infection with bluetongue virus type 16(BTV16).In this study,the mRNA of the above four cytokines were detected in three sheep infected with BTV16 using the Real-time quantitative PCR method.At the same time,negative control sheep were set up,and the relative expression of mRNA were calculated based on 0 d mRNA.Meanwhile,virus antibody titers were detected and sheep body temperature was measured.The results showed that all the three sheep inoculated with BTV16 had different degrees of antibody and body temperature symptoms,the mRNA levels of the four cytokines increased significantly within 2-4 days after inoculation of the virus.Among them,the peak value of IFN-γwas 2.58-27.84 times,the peak value of IL-2 was 5.24-17.19 times,the peak value of IL-4 was 2.16-3.43 times,and the peak value of IL-10 was 15.78-48.77 times.There were significant individual differences in the increase amplitude,and all the four cytokines decreased gradually after being kept at a high level for about 6 d.The above parameters of control sheep fluctuated within the normal range with no significant difference(P>0.05).This study elucidated the growth and decline characteristics of IFN-γ,IL-2,IL-4 and IL-10 in sheep inoculated with BTV16 at the transcriptional level,providing references for further studies on the characteristics of BTV infection and the immune mechanism of host organism.