茶树CsBBX基因家族的鉴定与表达

Identification and expression analysis of the CsBBX gene family in tea plants

锌指蛋白是一类含有“手指状”结构域的蛋白质,其中B-BOX(BBX)蛋白在植物的生长发育及逆境胁迫响应中具有重要的作用.利用生物信息学方法,从茶树基因组中鉴定出31个CsBBX基因,全面分析其生物信息学特征,同时分析它们在干旱、盐胁迫和MeJA处理中的转录组数据.结果显示,CsBBX基因大小差异较大,开放阅读框在324-1746 bp之间,编码氨基酸长在107-581 aa之间.CsBBX的编码蛋白序列中含有保守的BOX1(B1)、BOX2(B2)和CCT结构域,可以分为B1B2+CCT(Ⅰ和Ⅱ)、B1+CCT(Ⅲ)、B1+B2(Ⅳ)和B1(Ⅴ)等5种类型.启动子顺式作用元件预测显示,CsBBX基因启动子中含有多种光响应元件,同时还富含多种与植物生长发育及逆境胁迫响应相关的顺式作用元件.CsBBX基因在叶片组织中具有较高的表达水平,且部分基因随着叶片成熟度的增加转录水平升高.干旱和盐胁迫条件下,大部分CsBBX基因的表达受抑制,而部分基因被诱导表达;大部分CsBBX基因的表达受MeJA诱导.因此推测CsBBX基因在茶树生长发育及抗逆响应中发挥了重要的功能.

Zinc finger proteins are a type of protein with finger-like domains.B-BOX(BBX)proteins play an important role in plant growth,development,and stress response.In this study,thirty-one CsBBX genes were identified from the tea plant genome using bioinformatic methods,and their characteristics and expression patterns were subsequently investigated.Our results showed that the CsBBX genes exhibited large differences in sequence size,with open reading frames between 324 and 1746 bp,and coding amino acid lengths between 107 and 581 AA.The encoded protein sequences contained conserved BOX1(B1),BOX2(B2),and CCT domains.These proteins could,thus,be classified into the following five groups:B1B2+CCT(Ⅰ and Ⅱ),B1+CCT(Ⅲ),B1+B2(Ⅳ),and B1(Ⅴ).Promoter cis-acting element prediction showed that CsBBX genes not only contain a variety of light-responding elements,but are also enriched in various cis-elements associated with plant growth,development,and stress responses.CsBBX genes had a high expression level in leaf tissues,and the transcription levels of several genes increased with leaf maturity.Additionally,the expression of most CsBBX genes was repressed under drought conditions and in response to salt treatments,although several genes were upregulated.CsBBX genes were mainly induced by MeJA treatment.Our results indicated that CsBBX genes are involved in tea plant growth,development,and stress response.