皮肤再生医疗技术对糖尿病大鼠创面愈合及血管生成的影响

Effect of skin regeneration medical technology on wound healing and angiogenesis in diabetic rats

目的观察皮肤再生医疗技术(moist exposed burn therapy/moist exposed burn ointment,MEBT/MEBO)对糖尿病溃疡创面愈合及血管生成的影响,探讨MEBT/MEBO促进糖尿病创面愈合的机制。方法SPF级Wistar雄性大鼠24只,STZ注射建立糖尿病大鼠模型后随机分为对照组及MEBO组,各12只,背部切除建立创面,对照组创面给予生理盐水外敷治疗,MEBO组创面给予湿润烧伤膏(moist exposed burn ointment,MEBO)外敷治疗。于治疗第5天、第11天观察创面愈合情况及病理变化,行微血管计数,并采用免疫荧光法检测创面组织CD31水平。结果第5天,两组创面愈合率比较差异无统计学意义(P>0.05),第11天,MEBO组创面愈合率高于对照组(P<0.001);第5天,两组创面组织病理恢复情况及微血管计数比较差异无统计学意义(P>0.05),第11天,MEBO组创面病理恢复情况优于对照组,微血管计数高于对照组(P<0.001);血管标记物CD31显示第5天两组差异无统计学意义(P>0.05),第11天MEBO组明显高于对照组(P<0.001)。结论MEBT/MEBO可通过促进糖尿病创面新生血管形成而促进创面愈合。

Objective To observe the effect of moist exposed burn therapy/moist exposed burn ointment(MEBT/MEBO)on wound healing and angiogenesis of diabetic ulcers,and to explore the mechanism of MEBT/MEBO in promoting diabetic wound healing.Methods After establishing diabetic rat model by STZ injection,24 male Wistar rats of SPF grade were randomly divided into control group and MEBO group,with 12 rats in each group.The wounds in the control group were treated with saline by external application,and the wounds in the MEBO group were treated with MEBO by external application.And then,the wound healing and pathological changes were observed on the 5th and 11th day after treatment,microvessel count was performed,and CD31 level in wound tissue was detected by immunofluorescence method.Results On the 5th day,there was no statistically significant difference in wound healing rates between the two groups(P>0.05),on the 11th day,the wound healing rate of the MEBO group was higher than that of the control group(P<0.001);on the 5th day,there was no statistically significant difference in pathological recovery and microvessel count between the two groups(P>0.05),on the 11th day,the pathological recovery of the MEBO group was better than that of the control group,and the microvessel count was higher than that of the control group(P<0.001);the CD31 of vascular marker showed no statistically significant difference between the two groups on the 5th day(P>0.05),but on the 11th day,the MEBO group was significantly higher than the control group(P<0.001).Conclusion MEBT/MEBO can promote wound healing by promoting neovascularization of diabetic wound.