大孔吸附树脂纯化紫茶总多酚的工艺优化研究

Optimization of total polyphenol purification from purple tea by macroporous resin

目的筛选适合分离和纯化紫茶总多酚的大孔吸附树脂,确立其纯化工艺参数,以期制备出高纯度的紫茶总多酚,为其进一步开发奠定基础。方法采用静态吸附-洗脱试验筛选纯化紫茶总多酚的大孔吸附树脂,在单因素实验基础上,以吸附率等为指标,考察上样液质量浓度、上样液pH值、径高比、上样体积、上样体积流量、洗脱液乙醇体积分数、洗脱液体积以及洗脱体积流量对纯化工艺的影响,并确定最佳纯化工艺参数。结果AB-8型大孔吸附树脂纯化紫茶总多酚的最佳工艺参数为上样液质量浓度为375μg/ml,上样体积流量2 ml/min,上样体积3 BV,上样液pH为2,径高比为1∶6,洗脱时先用3 BV水去除杂质,再用50%乙醇4 BV洗脱,洗脱体积流量2 ml/min。结论AB-8型大孔吸附树脂可纯化紫茶总多酚,在最佳纯化工艺参数下,紫茶总多酚的质量分数从40.20%升至平均69.8%,干膏量由56.0 mg减少至29.9 mg,建立的工艺稳定、可行,可作为紫茶总多酚的纯化工艺条件。

Objective To screen the macroporous adsorption resin suitable for the separation and purification of total polyphenols from purple tea and establish the purification process parameters to prepare high-purity total polyphenols from purple tea.Methods The static adsorption-elution test was used to screen macroporous adsorption resin for the purification of total polyphenols from purple tea.Based on the single factor test,the comprehensive score of adsorption rate was used as the index to investigate the effects of different factors on the purification process and identify the optimal parameters for the purification process.Those factors included sample concentration,the pH value of the sample solution,the ratio of column diameter to height,sample size,ethanol percentage in the eluent,eluent volume and elution flow rate.Results The best process parameters for purification of total polyphenols from purple tea by AB-8 macroporous adsorption resin were as following.The sample concentration was 375μg/ml with flow rate 2 ml/min.The sample volume was 3 BV.The sample solution pH was 2.The ratio of colume diameter to height was 1∶6.The impurities were removed first by water 3 BV.50%ethanol 4 BV was used for elution with flow rate 2 ml/min.Conclusion AB-8 macroporous resin was selected for the purification of polyphenols from purple tea under the optimized technological conditions.The mass fraction of total polyphenols increased from 40.2%to an average of 69.8%.The solid content decreased from 56.0 mg to 29.9 mg.The established purification process has good stability and feasibility.It can be used as a purification process for total polyphenols from purple tea.