不同配比赤芍-桃仁乙醇提取物对急性血瘀模型大鼠血液流变学的影响

Effects of Different Proportions of Ethanol Extracts from Radix Paeoniae Rubra-Peach Kernel on Hemorheology in Acute Blood Stasis Rat

目的比较不同配比赤芍-桃仁乙醇提取物对急性血瘀模型大鼠血流变化的影响。方法选取赤芍-桃仁(1:0,0:1,1:1,1:2,2:1)配伍提取物,灌胃给予急性血瘀模型大鼠,通过测定全血黏度(WBV)、血浆黏度(PV)、红细胞压积(HCT)、红细胞沉降率(ESR),考察赤芍-桃仁不同配伍比例对大鼠血液流变学的影响;通过测定凝血酶原时间(PT)、纤维蛋白原(FIB)、凝血酶时间(TT),考察赤芍-桃仁不同配伍比例对凝血功能的影响。结果与空白对照组比较,模型对照组WBV、PV、HCT、ESR均显著升高(P<0.05),提示造模成功;与模型对照组比较,赤芍-桃仁(1:0)组、赤芍-桃仁(0:1)组的血流变学和凝血功能相关指标没有显著性改变;赤芍-桃仁(1:1)组的WBV、PV、HCT、ESR、FIB均显著降低(P<0.05),PT、TT显著延长(P<0.05);赤芍-桃仁(1:2)组的WBV、ESR、FIB显著降低(P<0.05),TT显著延长(P<0.05);赤芍-桃仁(2:1)组ESR显著升高(P<0.05),TT明显延长(P<0.01)。结论赤芍-桃仁不同配比对急性血瘀大鼠模型的血流流变学均有不同程度的改善,其中1:1组有极显著的作用,揭示两者配伍可能发生协同增效作用。

Objective To compare effects of different compatibility ratios of Radix Paeoniae Rubra-Peach Kernel on blood flow changes in rats with acute blood stasis.Methods The effects of different ratio of Radix Paeoniae Rubra-Peach Kernel(1:0,1:1,1:2,2:1,0:1)on hemorheology in rats with acute blood stasis were studied by measuring whole blood viscosity(WBV),plasma viscosity(PV),hematocrit(HCT),and erythrocyte sedimentation rate(ESR).The effects of different compatibility ratios of Radix Paeoniae Rubra and Peach Kernel on coagulation function were investigated by proenzyme time(PT),fibrinogen(FIB)and thrombin time(TT).Results Compared with the blank control group,WBV,PV,HCT,ESR in the model control group increased significantly(P<0.05),suggesting that the model was successful.Compared with the model control group,the hemorheology and coagulation function of the single drug group of Radix Paeoniae Rubra and Peach Kernel had no significant changes.WBV,PV,HCT,ESR,FIB of Radix Paeoniae Rubra-Peach Kernel(1:1)decreased significantly(P<0.05),and PT and TT were significantly prolonged(P<0.05).WBV,ESR,FIB of Radix Paeoniae Rubra-Peach Kernel(1:2)decreased significantly(P<0.05),and TT was prolonged significantly(P<0.05).Radix Paeoniae Rubra-Peach Kernel(2:1)increased ESR significantly(P<0.05),and TT was prolonged significantly(P<0.01).Conclusion Different ratios of Radix Paeoniae Rubra-Peach Kernel can improve the hemorheology of acute blood stasis rat model in different degrees,and the 1:1 ratio has a very significant effect,revealing that the compatibility of the two may have synergistic effect.