种植体周围炎龈沟液中IL-1β、IL-6和TNF-α的表达

Expression of IL-1β,IL-6 and TNF-αin sulcular fluid of peri-implantitis

目的·检测白介素(interleukin,IL)-1β、IL-6和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)在种植体周围炎患者龈沟液中的表达并分析其临床意义。方法·选择因牙列缺损行种植修复的患者24例,其中种植周围炎组患者12例,种植体周围组织健康组12例;另选择12名具健康天然牙的健康者为对照组。收集3组患者龈沟液,采用酶联免疫吸附法检测龈沟液中IL-1β、IL-6和TNF-α含量,分析其表达水平与龈沟液量和牙周探诊深度的相关性。结果·种植体周围炎组患者IL-1β、IL-6和TNF-α明显高于对照组(均P<0.05),且种植体周围炎组IL-1β和IL-6表达水平显著高于种植体周围组织健康组(均P<0.05);其中种植体周围炎组龈沟液中TNF-α表达水平与探诊深度呈正相关(r=0.600,P=0.039)。结论·种植体周围炎患者龈沟液中IL-1β、IL-6和TNF-α表达升高,且TNF-α表达水平与探诊深度呈正相关。

Objective·To investigate expressions of interleukin(IL)-1β,IL-6 and tumor necrosis factor-α(TNF-α)in sulcular fluid of peri-implantitis.Methods·Twenty-four patients with implant restoration were selected,including 12 patients in the peri-implantitis group and 12 patients in the periimplant health group.Twelve healthy persons with healthy gingival tissues were taken as the control group.The levels of IL-1β,IL-6 and TNF-αin gingival crevicular fluid were detected by enzyme-linked immunosorbent assay.The correlations between the expressions of these cytokines and the amount of gingival crevicular fluid or the probing depth were analyzed.Results·The levels of IL-1β,IL-6 and TNF-αin the peri-implantitis group were significantly higher than those in the control group(all P<0.05).The expression levels of IL-1βand IL-6 in the peri-implantitis group were significantly higher than those in the peri-implant health group(both P<0.05).The level of TNF-αin the peri-implantitis group was positively correlated with the probing depth(r=0.600,P=0.039).Conclusion·The levels of IL-1β,IL-6 and TNF-αin gingival crevicular fluid are highly expressed in patients with peri-implantitis.The expression of TNF-αis positively correlated with the probing depth.