摘要
目的利用生物信息学技术分析CBX1在胃癌中的表达,探讨CBX1与胃瘤临床病理特征的相关性,阐明CBX1对胃瘤早期诊断和预后的影响。方法利用Oncomine数据库、GEPIA数据库分析CBX1在胃癌组织和正常胃组织中转录水平的表达差异;利用Western blot检测CBX1在正常胃黏膜细胞GES-1和胃癌细胞SGC7901中的蛋白表达差异;利用Human Protein Atlas网站分析CBX1在胃癌组织及正常胃组织中翻译水平的表达差异;利用UALCAN数据库及Kaplan Meier-Plotter在线分析工具,阐明CBX1高表达与胃癌患者病理参数及患者生存率之间的关系;利用GEPIA数据库寻找与CBX1有紧密作用关系的蛋白,利用STRING数据库,构建蛋白质互作(PPI)网络,Scytoscape软件对PPI可视化,并利用Metascape网站进行基因本体富集分析,Starbase网站进行京都基因与基因组百科全书通路富集分析。结果CBX1在胃瘤中高表达,且与胃瘤的肿瘤分级、分期相关;生存分析结果显示CBX1高表达与胃癌患者生存率密切相关;富集分析结果显示与CBX1相互作用的蛋白主要发挥组蛋白修饰、mRNA组装功能。结论CBX1在胃瘤中呈高表达,并与胃瘤多个病理性指标相关。
Objective To analyze the expression of Chromobox 1(CBX1)protein in gastric cancer by bioinformatics,to explore its relationship with the clinicopathological features of gastric cancer,and to elucidate the effect of CBX1 on the early diagnosis and prognosis of gastric cancer.Methods Oncomine database and GEPIA database were used to analyze the expression differences of CBX1 in gastric cancer tissues and normal gastric tissues.Differences in protein expression of CBX1 between normal gastric mucosal cell line GES-1 and gastric cancer cell line SGC7901 were detected by Western blot.The Human Protein Atlas website analyzed the expression differences of CBX1 in gastric cancer tissues and normal gastric tissues.UALCAN database and kaplan-mey-plotter online analysis tools were used to elucidate the relationship between high expression of CBX1 and pathological parameters and overall survival rate of gastric cancer patients.The GEPIA database was used to search for proteins with close functional relationship with CBX1,and the protein-protein interaction(PPI)network was constructed by using Scytoscape software,and the gene ontology enrichment analysis was carried out on Metascape website,while the Kyoto encyclopedia of genes and genomes pathway enrichment analysis was carried out on Starbase website.Results CBX1 highly expressed in gastric cancer and correlated with tumor grade and stage of gastric cancer.Survival analysis showed that high mRNA expression of CBX1 associated with poor overall survival rate in gastric cancer patients.Enrichment analysis showed that interact with CBX1 protein could participate in the histone modification,mRNA assembly process.Conclusion CBX1 highly expressed in gastric cancer and related to several pathologic indexes of gastric cancer.
作者
吴爽
张涛元
李俏
曹三成
祝撷英
WU Shuang;ZHANG Taoyuan;LI Qiao;CAO Sancheng;ZHU Xieying(Department of Clinical Laboratory,the Affiliated Children Hospital of Xi′an Jiaotong University,Xi′an,Shaanxi 710003,China;Department of Anesthesiology,Rizhao International Heart Hospital,Rizhao,Shandong 276800,China)
出处
《国际检验医学杂志》
CAS
2021年第1期70-74,共5页
International Journal of Laboratory Medicine
基金
陕西省社会发展科技攻关项目(2016SF-036)
西安交通大学基本科研业务费自由探索与创新-教师类项目(xzy012019129)。
