高山大黄、手掌参煎液抑杀菌有效成分提取条件优化

Optimization of Extraction Conditions of Bacteriostatic Active Components from Radix et Rhizoma Rhei and Radix Sophorae Miltiorrhizae

为研究确定高山大黄、手掌参水煎液抑杀菌有效成分的最佳提取条件,试验以高山大黄、手掌参为试验材料,采用正交试验法,研究高山大黄、手掌参水煎液在不同药物颗粒、浸泡时间以及药物配比对药物提取效果的影响,试验结果用极差、方差进行分析,最终确定最优提取条件。结果表明,最佳提取工艺为:药物颗粒为60目以上、浸泡时间1h、高山大黄:手掌参=2:1。3个试验因素中,对提取液浓度影响最大的是药物颗粒,其次是药物配比,浸泡时间影响甚微。

In order to obtain the best extraction conditions of the antibacterial active ingredients of rhubarb and gardenia water decoction,the present experiment used rhubarb and gardenia as the test materials,and used orthogonal test method to study the rhubarb and gardenia decoction in different drug particles.The effect of soaking time and drug ratio on the extraction effect of the drug,the test results were analyzed with range and variance,and the optimal extraction conditions were selected based on the comprehensive test results.The results show that the best extraction process is:drug particles above 20 mesh,soaking time 0.5 hours,rhubarb:gardenia=3:1.Among the three test factors,the drug particles have the greatest impact on the concentration of the extract,followed by the drug ratio,and the soaking time has little effect.