AAV-DJ病毒对H9C2细胞感染效果的研究

Analysis of AAV-DJ Virus Infection to H9C2

目的将AAV-DJ-EGFP转染至H9C2细胞中,探究AAV-DJ病毒对H9C2的感染能力,从而寻找出一种新型的安全的对H9C2细胞感染能力强的病毒载体。方法往H9C2细胞中分别加入一定量的AAV-DJ-EGFP病毒及AAV9-EGFP病毒,通过荧光显微镜观察荧光强度来评价两者对H9C2细胞的感染效率,并通过细胞明场照片及CCK-8(cell counting kit-8)试剂盒检测评价AAV-DJ病毒对H9C2细胞活性的影响。结果与AAV9病毒比较,AAV-DJ病毒对H9C2细胞具有更高的感染效率,以AAV-DJ病毒为载体的绿色荧光蛋白的表达以时间梯度和浓度梯度递增。且细胞明场照片及细胞毒性试验表明,与未转病毒组比较,感染AAV-DJ病毒后的H9C2细胞的活力无明显变化。结论AAV-DJ病毒能安全高效地感染H9C2细胞。

Objective To explorethe ability of AAV-DJ virus to infect H9C2,so as to find a new and safe virus vector with strong ability to infect H9C2 cells.Methods A certain amount of AAV-DJ-EGFP and AAV9-EGFP was added to H9C2 cell culture,followed by visualization of cell fluorescence intensity using fluorescence microscopy to evaluate the infection efficiency of these virus serotypes.The effects of AAV-DJ virus on the viability of H9C2 cells were evaluated by cell brightfield photos and cell counting kit-8(CCK-8)kit detection.Results AAV-DJ showed greater infection efficiency in cardiac H9C2 cells compared to that of AAV9,and AAVDJ-mediated EGFP expression increased in a dose and time-dependent manner.In addition,the bright-field photos of the cells and the cytotoxicity test showed that the viability of H9C2 cells infected with the AAV-DJ virus did not change significantly compared with the untransfected group.Conclusion AAV-DJ virus could safely and efficiently infect H9C2 cells.