无透明带卵母细胞胞浆内单精子注射后成功妊娠分娩一例

A Case of Successful Pregnancy and Delivery after ICSI Derived from Zona-Free Oocyte

在体外受精(IVF)实验室中,经常会遇到无透明带卵母细胞(ZFO)。大多数ZFO是操作过程中损坏造成的,而不是透明带自身产生异常的结果。本文报道1例透明带脱落后的裸卵经胞浆内单精子注射(ICSI)后囊胚移植单胎活产健康婴儿的临床资料,并对卵母细胞透明带的生物学作用、ZFO的临床利用价值进行文献复习和讨论。建议对于那些成熟且形态正常的ZFO,用ICSI方法授精并培养至囊胚期后进行移植或玻璃化冷冻保存,为不孕患者,特别是那些卵母细胞数量有限的患者提供宝贵的胚胎来源。

Zona-free oocytes(ZFO)are frequently encountered in in-vitro fertilization(IVF)laboratories.The majority of these ZFO are due to the damage during manipulation,rather than abnormal zona production.We reported data case of a single live birth healthy baby after blastocyst transplantation following ICSI insemination of a naked egg after zona pellucida shedding.The biological function of zona pellucida and the clinical value of ZFOs were reviewed and discussed.We suggest that those mature and morphologically normal ZFO may be rescued,fertilized with ICSI and then cultured to the blastocyst stage for subsequent transfer or cryopreservation,so as to provide valuable embryo sources for infertile patients,especially for those patients with limited oocytes.