高通量转录组测序技术在评价子宫内膜容受性中的应用价值

Application of High-Throughput Transcriptome Sequencing in Evaluation of Endometrial Receptivity

良好的子宫内膜容受性是胚胎植入的关键因素,容受期子宫内膜组织的基因表达与容受性密切相关。通过高通量转录组测序技术可以获得月经不同时期子宫内膜基因表达图谱以及调控基因表达的微小RNA(miRNA)和长链非编码RNA(lncRNA)序列,结合生物信息学分析,可以更好地了解容受期子宫内膜特异基因表达,并为其调控机制的研究提供技术支持,对于不孕症患者以及反复植入失败患者的诊治具有非常重要的临床应用价值。对高通量转录组测序在子宫内膜容受性及胚胎植入方面的相关研究进行了综述。

The good endometrial receptivity is a key factor for embryo implantation.The gene expression profile of endometrial tissue cells in the receptive period is closely related to the endometrial receptivity.High-throughput transcriptome sequencing technology can obtain gene expression profiles of the endometrium at different periods of menstruation,as well as microRNA(miRNA)and long non-coding RNA(lncRNA)sequences that regulate gene expression.Combined with bioinformatics analysis,these profiles are very helpful for us to understand the expression of specific genes in the endometrium during the receptivity period,and provide technical support for the mechanism study.This high-throughput method may be used for the diagnosis and treatment of patients with infertility and repeated implantation failure.This review summarized the research progress of high-throughput transcriptome sequencing in the evaluation of endometrial receptivity and embryo implantation.