2种升温方式对毛蚶呼吸代谢及免疫相关酶活性的影响

Effects of Two Warming Patterns on Respiratory Metabolism and Immune-related Enzyme Activities of Ark Shell Scapharca subcrenata

采用室内控温试验,设置15、19、23、27℃4个温度梯度,每个温度梯度分别设置急剧升温和缓慢升温2种方式,研究2种升温方式对毛蚶呼吸代谢及免疫相关酶活性的影响。试验结果显示,在试验温度范围内,2种升温方式下毛蚶的耗氧率和排氨率均随海水温度的升高先增后降,于23℃达到最大值。2种升温方式下毛蚶耗氧率Q10值和排氨率Q10值在19~23℃最大,毛蚶O∶N均在19℃最小。急剧升温方式下,27℃处理组毛蚶肝胰腺中超氧化物歧化酶和过氧化氢酶的活性均呈先升后降的变化趋势,并在6 h达到峰值(P<0.05);23℃和27℃处理组毛蚶肝胰腺中酸性磷酸酶和溶菌酶活性的变化趋势均为先升后降,于6 h达到峰值(P<0.05)。2种升温方式相比,急剧升温比缓慢升温对毛蚶的影响更加明显。研究结果将有助于明确温度变化对毛蚶生理和生化活动的影响机制,为毛蚶的养殖生产提供依据。

The effects of changes in temperature on physiological and biochemical activities of ark shell Scapharca subcrenata were studied in a laboratory.Ark shell was held at sea water temperature of 15℃for 2 weeks and then divided into two groups:in one group the ark shell was directly moved into a tank at 19℃,23℃and 27℃,and in the other group the ark shell was slowly exposed to the same temperature at a warming rate of 1℃/12 h.It was found that the oxygen consumption rates and ammonia excretion rates of ark shell were increased first and then decreased with the increase in seawater temperature within the experimental temperature,with the maximal values at 23℃.The ark shell had the maximal oxygen consumption Q10 and ammonia excretion Q10 at 19—23℃,and had the minimal O∶N ratio at 19℃,under the rapid and slow warming patterns.Under the rapid warming pattern,activities of catalase(CAT),superoxide dismutase(SOD),acid phosphatase(ACP),and lysozyme(LSZ)were increased at first and decreased afterwards in ark shell exposed to high temperature(23℃or 27℃)for 96 h and reached the maximum at 6 h(P<0.05).There was more obvious effect on physiological and biochemical activities of ark shell under sudden warming than under slow warming.The findings will help to clarify the mechanism of temperature changes on physiological and biochemical activities of ark shell and provide reference data for the cultivation and production of ark shell.