睡眠限制引起的生物节律紊乱对大脑警觉度影响的研究

Effect of Biological Rhythm Disorder Induced by Sleep Restriction on Brain Alertness

目的通过大脑功能和脑电的变化探究睡眠限制引起的生物节律紊乱对大脑警觉度的影响。方法使用睡眠限制方法构建生物节律紊乱模型,10名受试者在6天内接受3次警觉度任务(PVT)实验并记录其脑电:前2天生物节律正常,此时定为基准;随后2天内比前2天延迟3h睡眠,此时生物节律紊乱;最后2天正常作息,生物节律恢复。统计PVT结果,从大脑皮层监测的脑电中提取事件相关谱扰动(ERSP),用大脑各个区域对应为节点,建立脑网络,提取节点的度、平均路径长度及聚类系数特征。结果PVT反应时间显示出警觉度的波动变化,ERSP显示额区、顶区、枕区4~10Hz脑电活跃,恢复期13~22Hz活跃,脑网络显示大脑功能性连接程度在紊乱期低,恢复期未恢复至基准水平。结论警觉度具有节律性,睡眠限制会引起有机生命体生物节律紊乱,警觉度下降。正常作息后警觉度又会有一定程度的恢复,但短期内无法回到初始水平。

Objective To explore the effects of biological rhythm disorder caused by sleep restriction on brain alertness from the changes of brain function and EEG.Methods The biological rhythm disorder model was constructed with sleep restriction method.Three times of vigilance task(PVT)experiments were carried out in 10subjects within 6days and the EEG was recorded.The biological exploration rhythm was normal in the first two days and it was set as the benchmark.Then in the next two days,the sleep was delayed for 3hours as compared with the first two days,and this time was the biological rhythm disorder period.After that,the biological rhythm was restored to normal work and rest for two days.The PVT results were counted and the event-related spectrum disturbance(ERSP)was extracted from the EEG monitored by the cerebral cortex.The brain network was established by using the corresponding brain regions as nodes to extract the degree,average path length and clustering coefficient characteristics of the nodes.Results The response time of PVT demonstrated the fluctuation of alertness.ERSP showed 4~10Hz EEG activity in frontal area,parietal area and occipital area,and 13~22Hz activity in recovery period.Brain network showed that the degree of brain functional connection was low in disorder period,and did not return to the baseline level in recovery period.Conclusion The alertness is rhythmic.Sleep restriction will cause the biological rhythm disorder in the organism,and the alertness will decrease.After normal work and rest,the alertness will recover to a certain extent,but it will not return to the initial level in short term.