柱层析纯化甜菊糖苷工艺技术

Purification of Stevia Glycoside by Column Chromatography

试验探索甜菊糖苷层析技术纯化的工艺条件。通过比较吸附剂种类、用量与洗脱剂浓度、用量等因素对甜菊糖苷提取率和纯度影响,研究适宜甜菊糖苷层析技术纯化的优化工艺条件。结果表明,用酸性氧化铝(或氧化镁)4 g粉末干法装柱、径高比1︰5、上样量3 mL、用70%的乙醇进行洗脱、10倍柱体积洗脱、回收乙醇,干燥得白色粉末状甜菊糖苷样品,纯度可达92.21%。酸性氧化铝(或氧化镁)层析技术用于甜菊糖苷纯化工艺流程更简单,且脱色效果好,产品纯度高。但氧化镁层析耗时较长,有待进一步改进提升。

To explore the purification technology of stevia glycoside chromatography,the extraction rate and purity of stevia glycosides were studied by comparing the adsorbent type,dosage,eluent concentration,dosage and other factors.The results showed that acid alumina(magnesium oxide)4 g powder dry method was used to load the column,the ratio of diameter to height 1︰5,the loading volume 3 mL,eluted with 70%ethanol,10 times the column volume eluted,the ethanol recovered,and the white powder stevia glucoside sample dried,and the purity was up to 92.21%.Conclusion acid alumina(magnesium oxide)chromatography was simpler for the purification of stevia glycosides,and the decolorization effect was better.However,magnesium oxide chromatography was time-consuming and needed further improvement.