溶血对冷球蛋白定量检测的影响

Effect of hemolysis on quantitative detection of cryoglobulin

目的:探讨溶血现象对冷球蛋白定量检测结果的影响。方法:选取2019年11月至2019年12月间冷球蛋白检测的患者20例,静脉采集2份血标本,1份进行人工溶血作为溶血样本,另1份作为对照样本,对2类样本进行冷球蛋白定量检测,比较2类样本的检测结果。结果:溶血组冷球蛋白中位数为246.63 mg/L,对照组的中位数为85.83 mg/L,溶血样本的冷球蛋白定量检测结果明显高于对照样本定量结果,差异有统计学意义(P<0.01);差值与对照组冷球蛋白数值呈中度相关性(r=0.56,P=0.01)。结论:标本溶血可导致冷球蛋白定量检测数值升高,升高程度与冷球蛋白含量存在一定相关性,为保证检测结果的准确性,应积极预防标本溶血。

Objective:To explore effect of hemolysis on quantitative detection of cryoglobulin.Methodology:From November,2019 to December,2019,20 patients were involved in this study.Blood sample was collected intravenously and divided into two copies.One sample was artificially hemolyzed as an hemolytic sample,the other was used as a control sample to quantitatively detect cryoglobulins in two types of samples.The results of two types of samples were compared.Results:The median cryoglobulin of hemolysis group was 246.63 mg/L,and the median cryoglobulin of control group was 85.83 mg/L.The results of cryoglobulin quantitative detection in observed samples were significantly higher than those in control samples(P<0.01).There was a moderate correlation between the difference and cryoglobulin values of control group(R=0.56,P=0.01).Conclusion:Hemolysis of blood sample can lead to the increase of cryoglobulin quantitative detection results.There was a certain correlation between the cryoglobulin content and the elevation degree.In order to ensure the accuracy of test results,we should prevent hemolysis of blood sample according to operating instructions.