马铃薯DRO1基因的克隆和逆境响应分析

Gene cloning and stress response analysis of DRO1 in potato

为探究马铃薯(Solanum tuberosum)深根基因DRO1的转录表达特性与功能,本研究克隆了该基因并对其进行了生物信息学预测分析。挑选了3个抗逆性不同的马铃薯品种‘大西洋’(Atlantic, Atl)、C119和C16,进行模拟干旱、高盐、低氮、低磷以及生长素和油菜素内酯6个不同处理,测定了试管苗的表型和生理指标以及StDRO1基因的相对表达量。结果表明,在6个不同处理下StDRO1基因表达量与对照相比均有升高,其中在干旱、生长素和低磷处理下的相对升高量较高。经相关性分析得到,干旱胁迫下的StDRO1基因相对升高量与SOD活性的相对变化呈显著正相关(P<0.05),而与株高和叶绿素含量的相对变化呈显著负相关(P<0.05);低磷处理下的StDRO1基因相对升高量与SOD的相对变化之间呈显著正相关(P<0.05),而与叶片数的相对变化显著负相关(P<0.05);生长素处理下的StDRO1基因相对升高量与根长的相对变化显著负相关(P<0.05),说明StDRO1基因强烈响应生长素以及干旱和低磷的逆境胁迫,并参与调控株高、根长、叶绿素含量和SOD活性。

In order to explore the characteristics and functions of StDRO1 in potato(Solanum tuberosum), StDRO1 was cloned and conducted with bioinformatic analysis. Then three potato ‘varieties Atlantic’(Atl), C119, and C16 with different stress resistance were selected, and six different treatments including drought, salt, low nitrogen, low phosphorus, auxin, and brassinolide were carried out. The results showed that the expression of StDRO1 was increased under all six different treatments compared to control, and the increase of StDRO1 expression was higher under drought, auxin and low phosphorus treatment. Correlation analysis showed that the increase of StDRO1 expression was significantly positively correlated with the relative change of SOD activity under drought stress(P<0.05), but negatively correlated with the relative changes of plant height and chlorophyll content(P<0.05). There was a significant positive correlation between the increase of StDRO1 expression and the relative change of SOD activity under low phosphorus treatment(P<0.05), but a significant negative correlation with the relative change of leaf number(P<0.05). Moreover, there was a significant negative correlation between the increase of StDRO1 expression and the relative change of root length(P<0.05), indicating that StDRO1 strongly responded to auxin, drought and low phosphorus stress, and might involve in regulation of plant height, root length, chlorophyll content, and SOD activity.