苦荞麦不同部位代谢轮廓及活性成分分布

Metabolic Profiling and Distribution of Bioactive Components of Different Parts of Tartary Buckwheat Seeds

应用气相色谱-质谱联用法对苦荞麦种子不同部位进行代谢轮廓分析,测定其不同部位的氨基酸、糖类、脂肪酸甲酯及游离脂肪酸的含量,并应用高效液相色谱法同时测定苦荞籽粒中芦丁、槲皮素、香草酸、对香豆酸、山柰酚及绿原酸6种活性成分的含量。结果表明,苦荞麦胚部代谢物的种类及含量均高于其他部位,总体上6种活性成分的含量在麸皮中高于其他部位,但芦丁在荞麦壳中的含量最高。

In this paper, gas chromatography-mass spectrometry(GC-MS) was used to analyze the metabolite profiles of different parts of tartary buckwheat seeds, and the contents of amino acids, sugars, fatty acid methyl esters and free fatty acids in these parts were determined. Six bioactive ingredients in tartary buckwheat seeds were simultaneously determined by high performance liquid chromatography(HPLC), including rutin, quercetin, vanillic acid, p-coumaric acid, kaempferol and chlorogenic acid. The results showed that the number and amount of metabolites in the germ were higher than those in the other parts tested. The contents of the six bioactive ingredients were higher in the bran than those in the other parts, but the content of rutin in the hull was the highest.