穴位埋线治疗非酒精性脂肪性肝病实验研究

Experimental Study on Catgut Embedment in Acupoint for Non-Alcoholic Fatty Liver Disease

目的:探讨穴位埋线法治疗非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)的作用。方法:将30只SD雄性大鼠随机分为对照组、模型组和埋线组,每组各10只。对照组以普通饲料喂养,模型组和埋线组均以高脂饲料喂养8周,复制NAFLD模型。模型复制成功后,埋线组以温脾化湿法为治疗原则,给予穴位埋线治疗,每周1次,连续治疗8周,模型组只放进固定器中15 min,不予埋线治疗,模型组和对照组在治疗后仍给予高脂饮食。检测各组大鼠血清谷丙氨酸氨基转移酶(alanine aminotransfease,ALT)、天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、三酰甘油(triglyceride,TG)、总胆固醇(triglyceride,TC),ELISA法检测大鼠血清视黄醇结合蛋白4(retinol binding protein,RBP4)水平。结果:与对照相比较,模型组和埋线组大鼠体质量、肝脏湿质量和肝脏质量指数显著升高(P<0.01)。与模型组比较,埋线组大鼠体质量、肝脏湿质量、肝脏质量指数下降(P<0.05)。与对照组比较,模型组大鼠血清ALT、AST、TG、TC、RBP4显著升高(P<0.01);与模型组比较,埋线组大鼠ALT、AST、TG、TC、RBP4显著下降(P<0.01)。结论:穴位埋线可以有效降低NAFLD大鼠血清异常升高的ALT、AST、TG、TC及RBP4水平,对NAFLD有一定治疗作用。

Objective:To explore the mechanism of catgut embedment in acupoint on non-alcoholic fatty liver disease(NAFLD).Methods:A total of 30 male SD rats were randomly divided into the control group,the model group and the catgut embedment group,with 10 rats in each group.The control group was fed with common feed,and the model group and the catgut embedment group were fed with high-fat diet for 8 weeks to replicate NAFLD model.After the model was successfully replicated,the catgut embedment group was given catgut embedment in acupoint therapy based on the treatment principle of warming spleen and resolving dampness,once a week for 8 weeks.The model group was only put into the fixator for 15 minutes without catgut embedment treatment.The model group and the control group were still given high-fat diet after the treatment.Serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglyceride(TG)and total cholesterol(TC)of each group were detected.The serum level of retinol binding protein 4(RBP4)was detected by ELISA.Results:Compared with the control group,the body weight,liver wet weight and liver mass index of the model group and the catgut embedment group were significantly higher,and all the differences were statistically significant(P<0.01).Compared with the model group,the body weight,liver wet weight and liver mass index in the catgut embedment group were significantly decreased,and all the differences were statistically significant(P<0.05).Compared with the control group,the serum ALT,AST,TG,TC of the model group were increased,and compared with the model group,the ALT,AST,TG,TC of the rats in the catgut embedment group were decreased,and all the differences were statistically significant(P<0.05).Compared with the control group,the serum RBP4 level in the model group and the catgut embedment group was increased,while compared with the model group,the RBP4 level in the catgut embedment group was lower than that in the model group,and all the differences were statistically significant(P<0.05).Conclusion:Catgut embedment in acupoint can effectively reduce the abnormal elevated levels of ALT,AST,TG,TC and RBP4 in NAFLD rats,which has a certain therapeutic effect on NAFLD.