延胡索、乌药、金铃子散、小活络丹对神经病理性疼痛镇痛作用的比较

Comparison of Analgesic Effects of Corydalis Yanhusuo,Radix Lindera,Jin Ling-zi powder and Xiao Huo-luo Dan on Rats with Neuropathic Pain

目的:比较延胡索、乌药、金铃子散、小活络丹对坐骨神经缩窄性损伤(chronic constriction injury,CCI)模型大鼠神经病理性疼痛(neuropathic pain,NP)的镇痛效果。方法:将SD雄性大鼠随机分为假手术组(n=13)、生理盐水组(n=13)、延胡索组(n=16)、乌药组(n=10)、金铃子散组(n=20)、小活络丹组(n=7),共6组。采用坐骨神经结扎法造模,于造模后第4天各组给予相应的药物(延胡索组、乌药组、金铃子散组、小活络丹组大鼠均按剂量12 g·kg-1给药,假手术组、生理盐水组给予等量生理盐水)灌胃,连续14天。从开始给药起,每隔1天测定其热痛阈值。结果:与生理盐水组比较,延胡索、乌药、金铃子散、小活络丹各组大鼠给药后热痛阈值均升高,金铃子散的效果稍微优于延胡索,乌药、小活络丹效果较差。结论:延胡索、乌药、金铃子散、小活络丹对神经病理性疼痛均具有一定的镇痛作用,其中金铃子散的镇痛作用较强且起效快,其次为延胡索,乌药和小活络丹对该种神经病理性疼痛的镇痛效果较不稳定。

Objective:To compare the analgesic effects of Corydalis Yanhusuo,Radix Lindera,Jin Ling-zi powder and Xiao Huo-luo Dan on chronic neuropathic pain in rats with sciatic nerve chronic constriction injury(CCI).Methods:The male SD rats were randomly divided into 6 groups,sham operation group(n=13),saline group(n=13),Corydalis Yanhusuo group(n=16),Radix Lindera group(n=10),Jin Ling-zi powder group(n=20)and Xiao Huo-luo Dan group(n=7),respectively.Four days after the CCI surgery,the drugs were given by intragastric administration.The rats in Corydalis Yanhusuo group,Radix Lindera group,Jin Ling-zi powder group and Xiao Huo-luo Dan group were given 12 g·kg-1 drug respectively.The sham operation group and saline group were given the same amount of saline.The rats were administered intragastrically once a day for 14 consecutive days.From the beginning of the administration,the threshold of thermal pain was measured every other day by hot needling instrument.Results:Compared with Control group,the thermal threshold of Corydalis Yanhusuo group,Radix Lindera group,Jin Ling-zi powder group and Xiao Huo-luo Dan group were all increased,but the increase of Corydalis Yanhusuo group and Jin Ling-zi powder group were more significantly.Conclusion:Corydalis Yanhusuo,Radix Lindera,Jin Ling-zi powder and Xiao Huo-luo Dan all have analgesic effect on neuropathic pain,among which Jin Ling-zi powder has the strongest analgesic effect and the fastest curative effect,followed by Corydalis Yanhusuo.The analgesic effects of Radix Lindera and Xiao Huo-luo Dan were not stable.