基于UHPLC-Q/TOF-MS技术的黄色金针菇菌柄和菌盖成分分析

UHPLC-Q/TOF-MS Analysis on Chemical Compounds in Stipe and Pileus of Flammulina filiformis

采用超高效液相色谱-四级杆串联飞行时间质谱法(UHPLC-Q/TOF-MS)对黄色金针菇(Flammulina filiformis)菌柄和菌盖化学成分进行分析。结果显示:黄色金针菇菌柄和菌盖代谢产物差异明显,得到8个差异代谢物,分别为L-瓜氨酸(L-citrulline)、α-亚麻酸(alpha-linolenic acid)、黄嘌呤(xanthosine)、2′-脱氧尿苷(2′-deoxyuridine)、吡哆醇(pyridoxine)、胸腺嘧啶(thymidine)、核糖胸腺嘧啶(ribothymidine)、烟酰胺(nicotinamide);根据保留时间、准分子离子、二级碎片裂解规律,再结合数据库信息,鉴定黄色金针菇中130种化合物,其中氨基酸及其衍生物33种、核苷酸及其衍生物34种、有机酸及脂类37种、糖醇类9种、生物碱类8种和其他化合物9种;呈味物质中,与菌柄比较,菌盖中水溶性糖醇含量较低,游离氨基酸和核苷酸含量较高。研究结果为黄色金针菇菌柄和菌盖代谢物差异、活性物质及呈味物质的研究提供参考。

Ultra high-performance liquid chromatography-mass spectrometry(UHPLC-Q/TOF-MS)was used to analyze chemical compounds in stipe and pileus of Flammulina filiformis.The results showed that there were significant metabolomic differences between stipe and pileus.Eight differential metabolites were identified,including L-citrulline,alpha linolenic acid,xanthosine,2′-deoxyuridine,pyridoxine,thymidine,ribozymidine,and nicotinamide.According to retention time,excimer ion,secondary fragment fragmentation pattern and comparison with compound databases,we identified 130 compounds,including 33 amino acids and their derivatives,34 nucleotides and their derivatives,37 organic acids and lipids,9 sugar alcohols,8 alkaloids,and 9 other compounds.For flavor compounds,the water-soluble sugar alcohol content in stipe was higher than that in pileus,whereas both free amino acid and nucleotide contents in pileus were higher than the free amino acid and nucleotide contents in stipe.These results provide a scientific basis for studying metabolomic difference,bioactive substances,and flavor components in F.filiformis.