Legiolert酶底物法与传统培养法对公共场所水体样本中嗜肺军团菌检出结果的比较

Comparison of the Legiolert enzyme-substrate method and the conventional cultivation for the detection of Legionella pneumophila in water samples from public places

目的比较Legiolert酶底物法与传统培养法对公共场所水体样本中嗜肺军团菌的检出效果。方法分别利用Legiolert酶底物法与传统培养法对采集于公共场所的冷却水、淋浴水样本进行嗜肺军团菌检测,并对分离株进行验证及血清分型。结果共采集并检测样品68份,传统培养法与Legiolert酶底物法的检出率分别为5.88%(4/68)及35.29%(24/68),差异有统计学意义(χ2=16.41,P<0.01)。两种方法的检测符合率为64.71%(44/68),检测效果的差异有统计学意义(配对χ2=16.41,P=0.000)。分离出的25株嗜肺军团菌血清型以LP1为主(14/25)。结论Legiolert酶底物法对公共场所水体样本中嗜肺军团菌的检出率高于传统培养法。

Objective To compare the detection effects of Legionella pneumophila in water samples from public places by the Legiolert enzyme-substrate method and the conventional cultivation.Methods The Legiolert enzyme-substrate method and the conventional cultivation method were used to detect Legionella pneumophila in cooling water and shower water samples collected in public places.Isolated strains were verified and serotyped.Results A total of 68 samples were collected and tested.The positive rate of the conventional cultivation and the Legiolert enzyme-substrate method were 5.88%(4/68)and 35.29%(24/68),respectively,with a significant difference(χ2=16.41,P<0.01).The coincidence rate of the two methods was 64.71%(44/68),and the difference of the detection effects of the two methods was statistically significant(χ2=16.41,P=0.000).A total of 25 strains of Legionella pneumophila were isolated,and the serum types were mainly LP1(14/25).Conclusion The Legiolert enzyme-substrate method represente a higher detection rate of Legionella pneumophila in water samples from public places than the conventional cultivation.